Abstract
Multiple shoots were induced from the cotyledonary nodes derived from seedling of Sesbania rostrata on Nitsch (1969; N) medium supplemented with various concentrations of benzyladenine (BA). 1 mg dm−3 BA proved to be the best, eliciting 5.8 ± 1.0 shoots per explant in 100 % cultures. The elongation of shoots was best at 2.0 mg dm−3 BA. The shoot proliferation capacity increased to 7.5 shoots per explant following transfer of explants to the fresh shoot multiplication medium (MS + 1.0 mg dm−3 BA), after an initial incubation of 30 d. To further enhance number of shoots per explant an alternative strategy of cultivation of mother explant on fresh shoot multiplication medium after excision of shoots was adopted. Following the repeated harvesting of shoots an average of 33 shoots per explant could be obtained. The in vitro regenerated shoots produced roots when transferred to half-strength MS medium supplemented with 3 % sucrose and 1 mg dm−3 IBA. The developed plantlets were planted in the soil and transferred to the field after an acclimatization period of 3 – 4 months. These plants produced flowers and fruits in the field and exhibited the development of prominent and more organized stem nodules as compared to the in vivo raised plants of the same age.
References
Jain, N., Babbar, S.B.: Recurrent production of plants of black plum, Syzygium cuminii (L.) Skeels, a myrtaceous fruit tree, from in vitro cultured seedling explants.-Plant Cell Rep. 19: 519-524, 2000.
Jha, A.K., Prakash, S., Jain, N., Nanda, K., Gupta, S.C.: Micropropagation of Sesbania sesban from the mature tree-derived explants.-Biol. Plant. 47: 121-124, 2003/4.
Kavierappa, K.M., Phillips, L.M., Trigiano, R.N.: Micropropagation of flowering dogwood (Cornus florida) from seedlings.-Plant Cell Rep. 16: 485-489, 1997.
Khattar, S., Mohan Ram, H.Y.: Organogenesis and plantlet formation in vitro in Sesbania grandiflora (L.) Pers.-Indian J. exp. Biol. 21: 251-253, 1983.
Kwon, D.K., Beevers, H.: Growth of Sesbania rostrata (Berm.) with stem nodules under controlled conditions.-Plant Cell Environ. 15: 939-943, 1992.
Mittal, A., Agrawal, R., Gupta, S.C.: In vitro development of plants from axillary buds of Acacia auriculiformis-a leguminous tree.-Plant Cell Tissue Organ Cult. 19: 65-70, 1989.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473-479, 1962.
Nitsch, J.P.: Experimental androgenesis in Nicotiana.-Phytomorphology 19: 398-404, 1969.
Vlachova, M., Meta, B.A., Schnell, J., de Bruijn, F.J.: The tropical legume Sesbania rostrata: tissue culture, plant regeneration and infection with Agrobacterium tumefaciens and rhizogenes strains.-Plant Sci. 50: 213-223, 1987.
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Jha, A., Prakash, S., Jain, N. et al. Micropropagation of Sesbania rostrata from the Cotyledonary Node. Biologia Plantarum 48, 289–292 (2004). https://doi.org/10.1023/B:BIOP.0000033458.88441.67
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DOI: https://doi.org/10.1023/B:BIOP.0000033458.88441.67