Abstract
Following 16, 40 and 64 h exposure to 0.33 M NaCl given after 8 h water imbibition, lentil seeds showed a gradual decrease of germination upon their transfer to water. These salt related changes were accompanied by modifications in the protein patterns of embryo axes as revealed by two-dimensional electrophoresis separation and by the computer image analysis of protein spots. In comparison with 8 h water imbibed seeds, prominent proteins comprised between the 5.1 – 7.6 pH isoelectric point in the first dimension and 75 – 50 kDa molecular mass in the second dimension showed a significant increase in their abundance as salt exposure increased. On transfer to water to complete germination, the content of many of these proteins decreased at 24h in 2 – 3 cm length embryo axes in comparison with the corresponding embryo axes of seeds continuously imbibed in water for 24 h. Some groups of proteins ranging between 15.5 – 17.3 kDa, already present after 8 h water imbibition, were not detectable after 24 h but were expressed in seeds exposed to NaCl and transferred to water for 24 h. Up- and down-regulated proteins in lentil embryo axes, imbibed under non-lethal salt stress conditions, have been tentatively identified by comparison with the protein map of germinating seeds of the model plant Arabidopsis.
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Bewley, J.D.: Seed germination and dormancy.-Plant Cell 9: 1055-1066, 1997.
Bliss, R.D., Platt-Aloia, K.A., Thomson, W.W.: Osmotic sensitivity in germinating barley seeds.-Plant Cell Environ. 9: 721-725, 1986.
Chen, R.D., Tabaeizadeh, Z.: Alteration of gene expression in tomato plants (Lycopersicon esculentum) by drought and salt stress.-Genome 35: 385-391, 1991.
Dell'Aquila, A.: Effect of combined salt and heat treatments on germination and heat-shock protein synthesis in lentil seeds.-Biol. Plant. 43: 591-594, 2000.
Dell'Aquila, A., Bewley, J.D.: Protein synthesis in the axes of polyethylene glycol treated pea seeds and during subsequent germination.-J. exp. Bot. 40: 1001-1007, 1989.
Dell'Aquila, A., Di Turi, M.: Amplification of ageing symptoms in two differently thermal-sensitive wheat (Triticum durum L.) genotypes by heat-shock: relationships between germination response and embryo protein patterns.-Seed Sci. Technol. 27: 467-476, 1999.
Dell'Aquila, A., Di Turi, M.: Two-dimensional electrophoresis and computer imaging analysis for the characterization of newly synthesized proteins in germinating durum wheat embryos.-Seed Sci. Technol. 30: 357-369, 2002.
Dell'Aquila, A., Spada, P.: Regulation of protein synthesis in germinating wheat embryos under polyethylene glycol and salt stress.-Seed Sci. Res. 2: 75-80, 1992.
Dell'Aquila, A., Spada, P.: The effect of salinity stress upon protein synthesis of germinating wheat embryos.-Ann. Bot. 72: 97-101, 1993.
Dell'Aquila, A., Corona, M.G., Di Turi, M.: Heat-shock proteins in monitoring aging and heat-induced toleance in germinating wheat and barley embryos.-Seed Sci. Res. 8: 91-98, 1998.
Dutta Gupta, S.: Protein profiles of somatic embryos and regenerated plants from NaCl selected and control cultures of orchardgrass.-Biol. Plant. 42: 297-302, 1999.
Elavumoottil, O.C., Martin, J.P., Moreno, M.L.: Changes in sugars, sucrose synthase activity and proteins in salinity tolerant callus and cell suspension cultures of Brassica oleracea L.-Biol. Plant. 46: 7-12, 2003.
Ellis, R.H., Roberts, E.H.: The quantification of ageing and survival in orthodox seeds.-Seed Sci. Technol. 101: 373-409, 1981.
Gallardo, K., Job, C., Groot, S.P.C., Puype, M., Demol, H., Vandekerckhove, J., Job, D.: Proteomic analysis of Arabidopsis seed germination and priming.-Plant Physiol. 126: 835-848, 2001.
Gulick, P.J., Dvorak, J.: Gene induction and repression by salt treatment in roots of the salinity-sensitive Chinese Spring wheat and the salinity-tolerant Chinese Spring x Elytrigia elongata amphiploid.-Proc. nat. Acad. Sci. USA 84: 99-103, 1987.
Kersten, B., Burkle, L., Kühn, E.J., Giavalisco, P., Konthur, Z., Lueking, A., Walter, G., Eickoff, H., Schneider, U.: Large-scale plant proteomics.-Plant. mol. Biol. 48: 133-141, 2002.
Ramagopal, S.: Differential mRNA transcription during salinity stress in barley.-Proc. nat. Acad. Sci. USA 84: 94-98, 1987.
Ramagopal, S.: Regulation of protein synthesis in root, shoot and embryonic tissues of germinating barley during salinity stress.-Plant Cell Environ. 11: 501-515, 1988.
Thiellement, H., Bahrman, N., Damerval, C., Plomion, C., Rossignol, M., Santoni, V., de Vienne, D., Zivy, M.: Proteomics for genetic and physiological studies in plants.-Electrophoresis 20: 2013-2026, 1999.
Waters, E.R., Garret, J.L., Vierling, E.: Evolution, structure and function of the small heat shock proteins in plants.-J. exp. Bot. 47: 325-338, 1996.
Zivy, M., de Vienne, D.: Proteomics: a link between genomics, genetics and physiology.-Plant mol. Biol. 44: 575-580, 2000.
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Dell'Aquila, A. Protein Patterns, Characterized by Computer Image Analysis, of Lentil Embryo Axes Germinating Under Salt Stress. Biologia Plantarum 48, 237–242 (2004). https://doi.org/10.1023/B:BIOP.0000033450.16985.07
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DOI: https://doi.org/10.1023/B:BIOP.0000033450.16985.07