Skip to main content
SpringerLink
Log in

Molecular Cloning and Different Expression of a Vacuolar Na+/H+ antiporter gene in Suaeda salsa Under Salt Stress

  • Published:
Biologia Plantarum

Abstract

A Na+/H+ antiporter catalyzes the transport of Na+ and H+ across the tonoplast membrane. We isolated a vacuolar Na+/H+ antiporter cDNA (SsNHX1) clone from a euhalophyte, Suaeda salsa. The nuclear sequence contains 2262 bp with an open reading frame of 1665 bp. The deduced amino acid sequence is similar to that of AtNHX1 and OsNHX1 in rice, with the highest similarities within the predicted transmembrane segments and an amiloride-binding domain. Northern blot analysis shows that the expression of the S. salsa gene was increased by salt stress. The results suggest that the SsNHX1 product is likely a Na+/H+ antiporter and may play important roles in the salt tolerance of S. salsa.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Apse, M.P., Aharon, G.S., Snedden, W.A., Blumwald, E.: Salt tolerance conferred by overexpression of a vacuolar Na+/H+ antiport in Arabidopsis.-Science 285: 1256-1258, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Barkla, B.J., Pantoja, O.: Physiology of ion transport across the tonoplast of higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 159-184, 1996.

    Article  PubMed  CAS  Google Scholar 

  • Barkla, B.J., Zingarelli, L., Blumwald, E., Smith, J.A.C.: Tonoplast Na+/H+ antiport activity and its energization by the vacuolar H+-ATPase in the halophytic plant Mesembryanthemum crystallinum L.-Plant Physiol. 109: 549-556, 1995.

    PubMed  CAS  Google Scholar 

  • Blumwald, E.: Tonoplast vesicles as a tool in the study of ion-transport at the plant vacuole.-Physiol. Plant. 69: 731, 1987.

    Article  CAS  Google Scholar 

  • Blumwald, E.: Sodium transport and salt tolerance in plants.-Curr. Opinion Cell Biol. 12: 431-434, 2000b.

    Article  PubMed  CAS  Google Scholar 

  • Blumwald, E., Aharon, G.S., Apse, M.P.: Sodium transport in plant cells.-Biochim. biophys. Acta 1465: 140-151, 2000a.

    Article  PubMed  CAS  Google Scholar 

  • Blumwald, E., Gelli, A.: Secondary in organic ion transport at the tonoplast.-Adv. Bot. Res. 25: 401, 1997.

    Article  CAS  Google Scholar 

  • Blumwald, E., Poole, R.J.: Na+/H+ antiport in isolated vesicles from storage tissue of Beta vulgaris.-Plant Physiol. 78: 163-167, 1985.

    PubMed  CAS  Google Scholar 

  • Chomczynski, P., Sacci, N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.-Anal. Biochem. 162: 156-159, 1987.

    Article  PubMed  CAS  Google Scholar 

  • Counillon, L., Pouyssegur, J., Reithmeier, R.: The Na+/H+ exchanger (NHE-1) contains N-and O-linked glycosyation restricted to the first N-terminal extracellular domain.-Biochemistry 33: 10463-10469, 1994.

    Article  PubMed  CAS  Google Scholar 

  • Dibrov, P., Fliegel, L.: Comparative molecular analysis of Na+/H+ exchangers: a unified model for Na+/H+ antiport?-FEBS Lett. 424: 1-5, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Flowers, T.J., Troke, P.F., Yeo, A.R.: The mechanism of salt tolerance in halophytes.-Annu. Rev. Plant Physiol. 28: 89-121, 1977.

    Article  CAS  Google Scholar 

  • Frommer, W.B., Ludewig, U., Rentsch, D.: Taking transgenic plants with a pinch of salt.-Science 285: 1222-1223, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Fukuda, A., Nakamura, A., Tanaka, Y.: Molecular cloning and expression of the Na+/H+ exchanger gene in Oryza sativa.-Biochim. biophys. Acta 1446: 149-155, 1999.

    PubMed  CAS  Google Scholar 

  • Gaxiola, R.A., Rao, R., Sherman, A., Grisafi, P., Alper, S.L., Fink, G.R.: The Arabidopsis thaliana proton transporters, AtNhxI and AvpI, can function in cation detoxification in yeast.-Proc. nat. Acad. Sci. USA 96: 1480-1485, 1999.

    Article  PubMed  CAS  Google Scholar 

  • Golldack, D., Dietz, K.J.: Salt-induced expression of the vacuolar H+-ATPase in the common ice plant is developmentally controlled and tissue specific.-Plant Physiol. 125: 1643-1654, 2001.

    Article  PubMed  CAS  Google Scholar 

  • Hahnenberger, K.M., Jia, Z., Young, P.G.: Functional expression of the Schizosaccharomyces pombe Na+/H+ antiporter gene, sod2, in Saccharomyces cerevisiae.-Proc. nat. Acad. Sci. USA 93: 5031-5036, 1996.

    Article  PubMed  CAS  Google Scholar 

  • Hasegawa, P.M., Bressan, R.A., Zhu, J.K., Bohnert, H.J.: Plant cellular and molecular responses to high salinity.-Annu. Rev. Plant Physiol. Plant mol. Biol. 51: 463-499, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Hofmann, K., Stoffel, W.: TM base-A database of membrane spanning proteins segments.-Biol. Chem. Hoppe-Seyler 347: 166, 1993.

    Google Scholar 

  • Jia, Z.P., Mcmullough, N., Martel, R., Hemmingsen, S., Young, P.G.: Nucleotide gene amplification at a locus encoding a putative Na+/H+ antiporter confers sodium and lithium tolerance in fission yeast.-EMBO J. 11: 1631-1640, 1992.

    PubMed  CAS  Google Scholar 

  • Munns, R., Greenway, H., Kirst, G.O.: Halotolerant eukaryotes.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Encyclopedia of Plant Physiology. Vol. 12. Part C. Pp. 59-135. Springer-Verlag, Berlin 1983.

    Google Scholar 

  • Nass, R., Cunningham, K.W., Rao, R.: Intracellular sequestration of sodium by a novel Na+/H+ exchanger in yeast is enhanced by mutations in the plasma membrane H+-ATPase.-J. biol. Chem. 272: 26145-26152, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Nass, R., Cunningham, K.W., Rao, R.: Novel localization of a Na+/H+ exchanger in a late endosomal compartment of yeast.-J. biol. Chem. 273: 21054-21060, 1998.

    Article  PubMed  CAS  Google Scholar 

  • Niu, X., Bressan, R.A., Hasegawa, P.M., Pardo, J.M.: Ion homeostasis in NaCl stress environment.-Plant Physiol. 109: 735-742, 1995.

    PubMed  CAS  Google Scholar 

  • Orlowski, J., Grinstein, S.: Na+/H+ exchangers of mammalian cell.-J. biol. Chem. 272: 22373-22376, 1997.

    Article  PubMed  CAS  Google Scholar 

  • Padan, E., Schuldiner, S.: Bacterial Na+/H+ antiporters: molecular biology, biochemistry, and physiology.-In: Konings, W.N., Kaback, H.R., Lolkema, J.S. (ed.): Handbook of Biological Physics. Vol. 2. Pp. 501-503. Elsevier Science, Amsterdam 1996.

    Google Scholar 

  • Ratajczak, R., Richter, J., Lüttge, U.: Adaptation of the tonoplast V-ATPase of Mesembryanthemum crystallinum to salt stress, C3-CAM transition and plant age.-Plant Cell Environ. 17: 1101-1112, 1994.

    Article  CAS  Google Scholar 

  • Sambrook, J., Fritsh, E.F., Maniatis, T.: Molecular Cloning: A Laboratory Manual. 2nd Ed.-Cold Spring Harbor Laboratory Press, Cold Spring Harbor 1989.

    Google Scholar 

  • Serrano, R., Mulet, J.M., Rios, G., Marquerz, J.A., Larrinoa, I.F., Leube, M.P., Mendizabal, I., Pascual-Ahuir, A., Proft, M., Ros, R., Montesinos, C.: A glimpse of the mechanisms of ion homeostasis during salt stress.-J. exp. Bot. 50: 1023-1036, 1999.

    Article  CAS  Google Scholar 

  • Shi, H., Ishitani, M., Kim, C., Zhu, J.K.: The Arabidopsis thaliana salt tolerance gene SOSI encodes a putative Na+/H+ antiporter.-Proc. nat. Acad. Sci. USA 97: 6896-6901, 2000.

    Article  PubMed  CAS  Google Scholar 

  • Very, A.A., Robinson, M.F., Mansfield, T.A., Sanders, D.: Guard cell cation channels are involved in Na+-induced stomatal closure in a halophyte.-Plant J. 14: 509-521, 1998.

    Article  CAS  Google Scholar 

  • Wang, B.S., Ratajczak, R., Zhang, J.H.: Activity, amount and subunit composition of vacuolar-type H+-ATPase and H+-PPiase in wheat roots under severe NaCl stress.-J. Plant Physiol. 157: 109-116, 2000.

    CAS  Google Scholar 

  • Zhang, L., Ma, X.L., Zhang, Q., Ma, C.L., Wang, P.P., Zhao, Y.X., Zhang, H.: Expressed sequence tags from a NaCl-treated Suaeda salsa cDNA library.-Gene 267: 193-200, 2001.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Key Laboratory of Plant Stress Research, College of Life Science, Shandong Normal University, Jinan, 250014, China

    X.-L. Ma, Q. Zhang, Y.-X. Zhao, C.-L. Ma & H. Zhang

  2. Department of Plant Sciences, University of Arizona, Tucson, AZ, 85721, USA

    H.-Z. Shi & J.-K. Zhu

Authors
  1. X.-L. Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Q. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. H.-Z. Shi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J.-K. Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Y.-X. Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. C.-L. Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. H. Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to H. Zhang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ma, XL., Zhang, Q., Shi, HZ. et al. Molecular Cloning and Different Expression of a Vacuolar Na+/H+ antiporter gene in Suaeda salsa Under Salt Stress. Biologia Plantarum 48, 219–225 (2004). https://doi.org/10.1023/B:BIOP.0000033448.96998.44

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BIOP.0000033448.96998.44

Search

Navigation