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Sequence Comparison of Plant Ornithine Decarboxylases Reveals High Homology and Lack of Introns

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Biologia Plantarum

Abstract

We have designed and constructed four oligonucleotides corresponding to the most conserved regions of ornithine decarboxylases (ODC; EC 4.1.1.17) of plant origin. These oligonucleotides were used for the amplification of homologous fragments from several plants (Zea mays, Capsicum annuum, Sorghum bicolor, Phaseolus vulgaris, Carica papaya and Daucus carota). The amplified fragments were cloned and sequenced, revealing high homology to other ODCs. Peptide sequences coded by these fragments were compared by Clustal analyses. These analyses identified the location of the conserved sequences corresponding to the binding sites of substrate and cofactor. Data demonstrated that the plant ODCs fragments lacked intron sequences and were extremely homologous (over 80 %), constituting a compact group separated from other eukaryotic ODCs.

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Authors and Affiliations

  1. Departamento de Biología Molecular, Instituto Potosino de Investigación Cientifíca y Tecnológica, Camino a la Presa de San José s/n, AP 3-74, 78210, Tangamanga, San Luis Potosí, México

    J.F. Jiménez-Bremont & A.P. Barba de la Rosa

  2. Departamento de Ingeniería Genética de Plantas, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, AP 629, 36500, Irapuato, Guanajuato, México

    Y.M. Camacho-Villasana, J.L. Cabrera-Ponce & N. Ochoa-Alejo

Authors
  1. J.F. Jiménez-Bremont
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  2. Y.M. Camacho-Villasana
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  3. J.L. Cabrera-Ponce
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  4. A.P. Barba de la Rosa
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  5. N. Ochoa-Alejo
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Correspondence to J.F. Jiménez-Bremont.

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Jiménez-Bremont, J., Camacho-Villasana, Y., Cabrera-Ponce, J. et al. Sequence Comparison of Plant Ornithine Decarboxylases Reveals High Homology and Lack of Introns. Biologia Plantarum 48, 193–198 (2004). https://doi.org/10.1023/B:BIOP.0000033444.17603.f2

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  • DOI: https://doi.org/10.1023/B:BIOP.0000033444.17603.f2

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