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Transformation of Tobacco Plants with cDNA Encoding Honeybee Royal Jelly MRJP1

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Biologia Plantarum

Abstract

For expression of MRJP1 - the most abundant protein of honeybee royal jelly - in plants, plasmid carrying the expression cassette composed of CaMV 35S RNA promoter, cDNA encoding MRJP1 with its native signal peptide, and nos3′ as transcription terminator in binary vector pBin19 was prepared. The plasmid was introduced into tobacco (Nicotiana tabacum L. cv. Wi38) plants by Agrobacterium tumefaciens-mediated transformation. Transgenic F1 and F2 generation was grown from the seeds of the primary obtained transgenic tobacco plants. Immunoblot analyses of protein leaf extracts from transgenic plants showed expression of MRJP1.

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Authors and Affiliations

  1. Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-845 38, Bratislava, Slovak Republic

    J. Júdová, R. Šutka, J. Klaudiny, D. Lišková & J. Šimúth

  2. USDA Plant Gene Expression Center, 800 Buchanan Street, Albany, CA, 947 10, USA

    D.W. Ow

Authors
  1. J. Júdová
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  2. R. Šutka
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  3. J. Klaudiny
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  4. D. Lišková
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  5. D.W. Ow
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  6. J. Šimúth
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Correspondence to J. Šimúth.

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Júdová, J., Šutka, R., Klaudiny, J. et al. Transformation of Tobacco Plants with cDNA Encoding Honeybee Royal Jelly MRJP1. Biologia Plantarum 48, 185–191 (2004). https://doi.org/10.1023/B:BIOP.0000033443.60872.f1

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  • DOI: https://doi.org/10.1023/B:BIOP.0000033443.60872.f1

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