Abstract
Shoot tip explants of cucumber (Cucumis sativus L. cv. Poinsett 76) were cultured in vitro on Murashige-Skoog medium with L-glutamine, ammonium nitrate, adenine sulphate, asparagine, ammonium succinate, potassium nitrate and sodium nitrate as the nitrogen sources along with optimal concentration of 0.044 mM benzyladenine to study their effects on in vitro morphogenesis. The explants grown with 0.068 mM L-glutamine displayed the highest culture response (74.6 %) and greatest shoot number per explant (13.6) at the end of two subcultures. The explants cultured with other nitrogen sources resulted in low culture frequency and low number of shoots per explant accompanied by basal callusing and necrosis.
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References
Chaleff, R.S.: Induction, maintenance, and differentiation of rice callus cultures on ammonium as sole nitrogen source.-Plant Cell Tissue Organ Cult. 2: 29-38, 1983.
Cousson, A., Tran Thauh Van, H.: Influence of ionic composition of the culture medium on de novo flower formation in tabacco thin cell layers.-Can. J. Bot. 71: 1506-1511, 1993.
Gamborg, O.L., Miler, R.A., Ojima, K.: Nutrient requirements of suspension cultures of soybean root cells.-Exp. Cell Res. 50: 151-158, 1968.
Gamborg, O. L.: The effects of amino acids and ammonium on the growth of plant cells in suspension culture.-Plant Physiol. 45: 372-375, 1970.
Gomez, K.A., Gomez, K.A.: Statistical Procedures for Agricultural Research with Emphasis of Rice.-Rice Res. Inst., Los Baños 1976.
Grimes, H.D., Hodges, T.K.: The inorganic NO3 -:NH4 + ratio influences plant regeneration and auxin sensitivity in primary callus derived from immature embryos of Indica rice (Oryza sativa L.).-Plant Physiol. 136: 362-367, 1990.
Halperin, W., Wetherell, D.E.: Ammonium requirement for embryogenesis in vitro.-Nature 205: 519-520, 1965.
Khanna, H., Raina, S.K.: Enhanced in vitro plantlet regeneration from embryo-derived primary callus of a Basmati rice cultivar through modification of nitrate -nitrogen and ammonium -nitrogen concentrations.-J. Plant Biochem. Biotechnol. 6: 85-89, 1997.
Locy, R.D., Wehner, T.C.: Cucumber shoot tip growth on 9 nitrogen sources in in vitro culture.-Cucurbits Genet. Coop. Rep. 5: 10-11, 1982.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Plant Physiol. 15: 473-497, 1962.
Nuutila, A.M., Hamalainen, J., Mannonen, I.: Optimization of media nitrogen and copper concentration for regeneration of green plants from polyembryogenic cultures of barley (Hordeum vulgare L.).-Plant Sci. 151: 85-92, 2000.
Preece, J.E.: Can nutrient salts partially substitute for plant growth regulators?-Plant Tissue Cult. Biotechnol. 1: 26-37, 1995.
Reinert, J.M., Tazawa, R., Semeroff, S.: Nitrogen compounds as factors of embryogenesis in vitro.-Nature 216: 1215-1216, 1967.
Selby, C., Harvey, B.M.R.: The influence of composition of the basal medium in the growth and morphogenesis of cultured Sitka spruce (Picea sitchensis) tissues.-Ann. Bot. 65: 395-407, 1990.
Selvaraj, N., Vengadesan, G., Vasudevan, A., Prem Anand, R., Ramesh Anbazhgan, V., Ganapathi, A.: Micropropagation of Cucumis sativus L. from field grown plants.-In: Mayhard, D.N. (ed.): Proceedings of Cucurbitaceae 2002 Conference. Pp. 149-156. Naples 2002.
Tazawa, M., Reinert, J.: Influence nitrogen sources by suspension cell cultures of carrot.-Protoplasma 68: 157, 1969.
Vasudevan, A., Selvaraj, N., Suresh Kumar, P., Ganapathi, A.: Multiple shoot induction from the shoot tip explants of cucumber (Cucumis sativus L.).-Cucurbits Genet. Coop. Rep. 24: 8-12, 2001.
Wetherell, D.F., Dougall, D.K.: Sources of nitrogen supporting growth and embryogenesis in cultured wild carrot tissue.-Plant Physiol. 37: 97-103, 1976.
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Vasudevan, A., Selvaraj, N., Ganapathi, A. et al. Glutamine: A Suitable Nitrogen Source for Enhanced Shoot Multiplication in Cucumis sativus L.. Biologia Plantarum 48, 125–128 (2004). https://doi.org/10.1023/B:BIOP.0000024288.82679.50
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DOI: https://doi.org/10.1023/B:BIOP.0000024288.82679.50