Abstract
An efficient in vitro plant regeneration system via hypocotyl segments of tetraploid Isatis indigotica Fort. was established. Murashige and Skoog's (MS) and Gamborg's (GB5) media were found to be superior to White medium for promoting shoot regeneration. The highest shoot regeneration (92 %) was achieved from hypocotyls cultured on MS medium containing 8.9 μM benzyladenine (BA) and 2.7 μM naphthaleneacetic acid (NAA), with an average of 4.2 shoots developed per explant. Plant regeneration was also improved when the explants were cultured in MS basal medium containing 3 % (m/v) sucrose and grown under a 12-h photoperiod. The developed shoots were well rooted in a half-strength MS medium supplemented with 0.5 μM indole-3-butyric acid (IBA) and were morphologically normal after transfer to soil.
References
Bischoff, A., Mahn, E.G.: The effects of nitrogen and diaspore availability on the regeneration of weed communities following extensification.-Agr. Ecosyst. Environ. 77: 237-246, 2000.
Chen, J.T., Chang, W.C.: Effects of tissue culture conditions and explant characteristics on direct somatic embryogenesis in Oncidium ‘Gower Ramsey’.-Plant Cell Tissue Organ Cult. 69: 41-44, 2002.
Chen, W., Du, L.Y., Cun, S.X., Zhao, F.P.: [Research on tissue culture from hypocotyl in Isatis indigotica].-J. Pharm. Pract. 18: 337-339, 2000. [In Chin.]
Gamborg, O.L.: The effects of amino acids and ammonium on the growth of plant cells in suspension culture.-Plant Physiol. 45: 372-375, 1970.
Gamborg, O.L.: Plant tissue culture. Biotechnology. Milestones.-In Vitro cell. dev. Biol. Plant 38: 84-92, 2002.
Hu, Q., Andersen, S.B., Hansen, L.N.: Plant regeneration from mesophyll protoplasts in Isatis indigotica.-Plant Cell Tissue Organ Cult. 55: 155-157, 1999.
Murashige, T., Skoog, F.A.: Revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 15: 473-479, 1962.
Pablo, E.V., Andrês, C.H., Ma, E.V., Octavio, P.L.: Plant regeneration via organogenesis in marigold.-Plant Cell Tissue Organ Cult. 69: 279-283, 2002.
Peng, F., Wang, F.A.: [Study on plant regeneration from hypocotyl of Isatis indigotica Fort. in vitro].-J. Hunan Agr. Coll. 20: 450-455, 1994. [In Chin.]
Qiao, C.Z., Dai, F.B., Cui, X.: [Pharmacognostical studies on the two ploidy level of indigowoad (Isatis indigotica)].-Chin. Trad. Herb Drugs 26: 423-429, 1995. [In Chin.]
Singh, A.K., Chand, S., Pattnaik, S., Chand, P.K.: Adventitious shoot organogenesis and plant regeneration from cotyledons of Dalbergia sissoo Roxb., a timber yielding tree legume.-Plant Cell Tissue Organ Cult. 68: 203-209, 2002.
Svetleva, D., Velcheva, M., Dimova, D., Ivanova, K., Petkova, S.: Factors influencing the in vitro cultivation of common bean (Phaseolus vulgaris L.) leaf petioles.-Biotech. biotech. Equip. 15: 28-34, 2001.
Tang, K., Hu, Q., Zhao, E., Wu, A.: Factors influencing plant regeneration from protoplasts isolated from long-term cell suspension culture of recalcitrant indica rice cultivar IR36.-In Vitro cell. dev. Biol. Plant 36: 255-59, 2000.
Vanegas, P.E., Cruz-Hernandez, A., Valverde, ME., Paredes-Lopez, O.: Plant regeneration via organogenesis in marigold.-Plant Cell Tissue Organ Cult. 69: 279-283, 2002.
Wang, Y., Qiao, C.Z., Liu, S., Zhang, HM.: [Evaluation on antiendotoxic action and antiviral action in vitro of tetraploid Isatis indigotica].-Chin. J. chin. Materia med. 25: 327-329, 2000. [In Chin.]
White, P.R.: Nutrient deficiency studies and an improved inorganic nutrient for culture of excised tomato roots.-Growth 7: 53-65, 1943.
Yokoya, N.S., Handro, W.: Effects of plant growth regulators and culture medium on morphogenesis of Solieria filiformis (Rhodophyta) cultured in vitro.-J. appl. Phycol. 14: 97-102, 2002.
Zheng, G.Z., Yang, Y.S., Chen, X.H., Wan, B.H.: Evaluation of a new culture method for mass-propagation of a photoperiod-temperature sensitive genic male sterile rice strain N19S.-Plant Cell Tissue Organ Cult. 68: 195-202, 2002.
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Zhang, L., Kai, G., Xu, T. et al. Efficient Regeneration of Tetraploid Isatis indigotica Plants via Adventitious Organogenesis from Hypocotyl Explants. Biologia Plantarum 48, 121–124 (2004). https://doi.org/10.1023/B:BIOP.0000024287.23065.e8
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DOI: https://doi.org/10.1023/B:BIOP.0000024287.23065.e8