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Characterisation of Soybean and Wheat Seeds by Nuclear Magnetic Resonance Spectroscopy

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Biologia Plantarum

Abstract

The effects of equilibration under different air relative humidities (RH, 1 – 90 %) and temperatures (35 and 45 °C) on soybean (Glycine max) and wheat (Triticum aestivum) seeds were studied using different techniques. Seed moisture content, electrical conductivity (EC) of seed leachate and per cent seed germination were measured following standard procedures, and compared with nuclear magnetic resonance spin-spin relaxation time (T2) measurements. Moisture contents of soybean and wheat seeds, following the reverse sigmoidal trend, were greater at 35 than at 45 °C at any particular RH. Changes in T2 were related to the changes in germination percentage and leachate EC of both soybean and wheat seeds. Equilibrating soybean seeds at RH ≤ 11 % decreased germination percentage with corresponding decrease in T2. On the contrary, EC of seed leachate increased. In wheat seeds equilibrated at 45 °C, T2 was maximal at RH 5.5 %. T2 declined in seeds equilibrated at high RH (> 80 %) together with low germination percentage.

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References

  • Brosio, E., Di Nola, A., Fracassi, M., Carnovale, E., Marconi, E.: NMR study of seed hydration: The role of the seed anatomical structures in water uptake of soaked cowpeas.-Cell. mol. Biol., 38: 693-697, 1992.

    Google Scholar 

  • Buitnik, J., Mireille, M.A., Classens, E., Marcus Hemminga, A., Hoekstra, F.A.: Influence of water content and temperature on molecular mobility and intra-cellular glasses in seeds and pollen.-Plant Physiol. 118: 531-541, 1998.

    Google Scholar 

  • Ellis, R.H., Hong, T.D., Roberts, E.H.: A comparison of the low-moisture content limit to logarithmic relation between seed moisture content and longevity in twelve species.-Ann. Bot. 63: 601-611, 1989.

    Google Scholar 

  • Fang, J., Moore, F., Roos, E., Walters, C.: Three-dimensional models represent seed moisture content as a function of relative humidity and temperature.-HortScience 33: 1207-1209, 1998.

    Google Scholar 

  • Fukuoka, M., Watanabe, H., Mihori, T., Shimada, S.: Moisture diffusion in a dry soybean seed measured using pulsed-field-gradient NMR.-J. Food Eng. 23: 533-541, 1994.

    Google Scholar 

  • ISTA: International rules for seed testing.-Seed Sci. Technol. 13: 299-513, 1985.

    Google Scholar 

  • Krishnan, P., Nagarajan, S., Moharir, A.V.: Changes in NMR relaxation times as a measure of membrane stability in seeds equilibrated at different temperature and relative humidity.-Indian J. Biochem. Biophys. 40: 46-50, 2003a.

    Google Scholar 

  • Krishnan, P., Nagarajan, S., Moharir, A.V.: Characterization of wheat (Triticum aestivum) and soybean (Glycine max) seeds under accelerated ageing conditions by proton nuclear magnetic resonance spectroscopy.-Seed Sci. Technol. 31: 541-550, 2003b.

    Google Scholar 

  • Millard, M.M., Veisz, O. B., Krizek, D., Line, M.: Thermodynamic analysis of physical state of water during freezing in plant tissue, based on the temperature dependence of proton spin-relaxation.-Plant Cell Environ. 19: 33-42, 1996.

    Google Scholar 

  • Noda, N., Sakaguchi, K., Kumamoto, Y., Iwaya Inoue, M.: Determination of the phase change in the H1-NMR relaxation behavior of dehydrating soybean seed using AIC method.-J. Fac. Agr. 43: 67-74, 1998.

    Google Scholar 

  • Ratcliffe, R.G.: In vivo NMR studies of higher plants and algae.-Adv. bot. Res. 20: 44-123, 1994.

    Google Scholar 

  • Ratcliffe, R.G., Shachar-Hill, Y.: Probing plant metabolism with NMR.-Annu. Rev. Plant Physiol. Plant mol. Biol. 52: 499-526, 2001.

    Google Scholar 

  • Snarr, J.E.M., Van As, H.: Probing water compartments and membrane permeability in plant cells by H1 NMR relaxation measurements.-Biophys. J. 63: 1654-1658, 1992.

    Google Scholar 

  • Vertucci, C.W., Roos, E.E.: Theoretical basis of protocols for seed storage II. Influence of temperature on optimum moisture levels.-Seed Sci. Res. 3: 201-213, 1993.

    Google Scholar 

  • Walters, C.: Understanding the mechanisms and kinetics of seed ageing.-Seed Sci. Res. 8: 223-244, 1998.

    Google Scholar 

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Authors and Affiliations

  1. Division of Agricultural Physics, Indian Agricultural Research Institute, New Delhi, 110012, India

    P. Krishnan & A.V. Moharir

  2. Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi, 110012, India

    D.K. Joshi, M. Maheswari & S. Nagarajan

Authors
  1. P. Krishnan
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  2. D.K. Joshi
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  3. M. Maheswari
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  4. S. Nagarajan
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  5. A.V. Moharir
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Correspondence to P. Krishnan.

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Krishnan, P., Joshi, D., Maheswari, M. et al. Characterisation of Soybean and Wheat Seeds by Nuclear Magnetic Resonance Spectroscopy. Biologia Plantarum 48, 117–120 (2004). https://doi.org/10.1023/B:BIOP.0000024286.23683.05

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  • DOI: https://doi.org/10.1023/B:BIOP.0000024286.23683.05

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