Abstract
Suckers collected from different populations of Musa acuminata ssp. malaccensis were found to be highly resistant to race 4 of Fusarium oxysporum f. sp. cubense (FOC) suggesting that local wild banana populations co-evolved with the pathogen. Seedlings from these wild banana plants segregated for resistance to the pathogen. The infected seedlings were characterized based on external and internal symptoms and the variable response to FOC was mainly due to the genetic factors. Using the technique of random amplified polymorphic DNA (RAPD), 96 major amplification products from 15 primers were identified. Only 10 out of 96 markers were monomorphic and shared among the seed progenies, whereas the remaining 86 were highly polymorphic. Three primers showed banding patterns specific to resistant or susceptible seedlings. These results showed the great potential of the wild Musa acuminata ssp. malaccensis as a source for banana improvement and also for the synthesis of segregating populations for linkage mapping, gene cloning and DNA markers related to FOC resistance.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asif, M.J., Mak, C., Othman, R.Y.: Characterization of indigenous Musa species based on flow cytometric analysis of ploidy and nuclear DNA content.-Caryologia 54: 161-168, 2001a.
Asif, M.J., Mak, C., Othman, R.Y.: Factors affecting the germination and seedling growth of in vitro grown zygotic embryos of a local wild banana (Musa acuminata ssp. malaccensis).-Plant Cell Tissue Organ Cult. 67: 267-270, 2001b.
Beckman, C.H.: Host responses to the pathogen.-In: Ploetz, R.C. (ed.): Fusarium Wilt of Banana. Pp. 93-106. APS Press, St. Paul 1990.
Beckman, C.H., Halmos, S., Mace, M.E.: The interaction of host, pathogen and soil temperature in relation to susceptibility to Fusarium wilt of bananas.-Phytopathology 52: 134-140, 1961.
Bhat, K., Jarret, R.L.: Random amplified polymorphic DNA and genetic diversity in Indian Musa germplasm.-Genet. Resour. Crop Evolut. 42: 107-118, 1996.
Brake, V.M., Pegg, K.G., Irwin, J.A.G., Chaseling, J.: The influence of temperature, inoculum level and race of Fusarium oxysporum f. sp. cubense on the disease reaction of banana cv. Cavendish.-Aust. J. agr. Res. 46: 673-685, 1995.
Buddenhagen, I.W.: Banana bleeding and Fusarium wilt.-In: Ploetz, R.C. (ed.): Fusarium Wilt of Banana. Pp. 107-113. APS Press, St. Paul 1990.
Damasco, O.P., Graham, G.C., Henry, R.J., Adkins, S.W., Smith, M.K., Godwin, I.D.: Random amplified polymorphic DNA (RAPD) detection of dwarf off-types in micropropagated Cavendish (Musa ssp. AAA) bananas.-Plant Cell Rep. 16: 118-123, 1996.
Doyle, J.J., Doyle, J.L.: A rapid DNA isolation procedure for small quantities of fresh leaf tissue.-Phytochem. Bull. 19: 11-15, 1987.
Gabriel, D.W., Loschke, D.C., Rolfe, B.G.: Gene for gene recognition: The ion channel difuse model.-In: Palacios, R., Verma, D.P. (ed.): Molecular-Plant Microbe Interactions. Pp. 3-14. APS Press, St. Paul 1988.
Gawel, N., Jarret, R.L.: Chloroplast DNA restriction fragment length polymorphism's (RFLPs) in Musa species.-Theor. appl. Genet. 81: 783-786, 1991.
Ho, H.Y.: The development of Pisang Mutiara-a Fusarium wilt tolerant Rastali.-In: Wahab, Z. (ed.): Proceedings of the First National Banana Seminar. Pp. 144-147. Awana-Genting, Pahang 1999.
Howell, E.C., Newbury, J.H., Swennen, R., Withers, L.A., Ford-Llyod, B.V.: The use of RAPD for identifying and classifying Musa germplasm.-Genome 37: 328-332, 1994.
Hwang, S., Ko, W.: Somaclonal variation of bananas and screening for resistance to Fusarium wilt.-In: Persley, G., De Langhe, E. (ed.): Banana and Plantain Breeding Strategies. Pp. 151-156. ACIAR, Canberra 1987.
Kaemmer, D., Fischer, D., Jarret, R.L., Baurens, F.C., Grapin, A., Dambier, D., Noyer, J.L., Lanuad, C., Kahl, G., Lagoda, P.J.L.: Molecular breeding in the genus Musa: a strong case for STMS market technology.-Euphytica 96: 49-63, 1997.
Mohamed, A.A., Mak, C., Liew, K.W., Ho, Y.W.: Early evaluation of banana plants at nursery stage for Fusarium wilt tolerance.-In: Molina, A.B., Nik Masdek, N.H., Liew, K.W. (ed.): Seminar on Banana Fusarium Wilt Management Towards Sustainable Cultivation. Pp. 174-185. Genting Highlands Resort, Genting Pahang 1999.
Orjeda, G., Escalant, J.V., Moore, N.: The International Musa Testing Program (IMTP) phase II. Overview of final report and summary of results.-InfoMusa 8: 3-10, 1999.
Ortiz, R., Vuylsteke, D.: Inheritance of albinism in banana and plantains (Musa spp.).-HortScience 29: 903-904, 1993.
Pegg, K., Moore, N., Bentley, S.: Fusarium wilt of bananas in Australia: A review.-Aust. J. agr. Res. 47: 637-650, 1996.
Ploetz, R.C., Vasquez, A., Nagel, J., Benscher, D., Sianglen, P., Srikul, S., Kooariyakul, S., Wattanachaiyingcharoen, W., Letrat, P., Wattanachaiyingcharoen, D.: Current status of Panama disease in Thailand.-Fruits 51: 387-395, 1997.
Rowe, P.R., Rosales, F.E.: Banana and plantains.-In: Janick, J., Moore, N. (ed.): Advances in Fruit Breeding. 2nd Ed. Pp. 167-211. Timber Press, Portland 1993.
Shepherd, K.: Banana breeding. Past and present.-Acta Hort. 196: 37-43, 1987.
Simmonds, N.W.: The Evolution of the Bananas.-Longman, London 1962.
Singleton, L.L., Mihail, J.D., Rush, C.M. Singleton, L.L., Mihail, J.D., Rush, C.M. (ed.): Methods for Research on Soil Borne Phytopathogenic Fungi.-APS Press, St. Paul 1992.
Stover, R.H., Buddenhagen, I.W.: Banana breeding, polyploidy, disease resistance and productivity.-Fruits 41: 175-191, 1986.
Tang, C.Y., Hwang, S.C.: Performance of banana clones under the challenge of Fusarium wilt in Taiwan.-InfoMusa 8: 10-12, 1999.
Vakili, N.G.: Fusarium wilt resistance in seedlings and mature plants of Musa species.-Phytopathology 55: 135-140, 1965.
Weising, K., Nybom, H., Wolff, K., Meyer, W. (ed.): DNA Fingerprinting in Plants and Fungi.-CRC Press, Boca Raton 1995.
Welsh, J., McClelland, M.: Fingerprinting genomes using PCR with arbitrary primers.-Nucl. Acids Res. 18: 7213-7218, 1990.
Williams, J.G.K., Kubelik, A.R., Livak, K.J., Rafalski, J.A., Tingey, S.V.: DNA polymorphism amplified by arbitrary primers are useful as genetic markers.-Nucl. Acids Res. 18: 6531-6535, 1990.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Javed, M., Chai, M. & Othman, R. Study of Resistance of Musa acuminata to Fusarium oxysporum using RAPD markers. Biologia Plantarum 48, 93–99 (2004). https://doi.org/10.1023/B:BIOP.0000024281.85427.6d
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000024281.85427.6d