Abstract
A protein, identifiable as calmodulin (CaM), has been isolated from the seedling tissue of Pharbitis nil. The method has been developed to isolate a high quality protein from plant tissue containing the high content of polyphenols. This protein was relatively heat-stable and bound to hydrophobic resin in calcium-dependent manner. It was recognized by the antibody against pea and carrot, but did not bind to antibody against Dictyostelium discoideum. This protein had Mr of 15 kDa and 18.5 kDa in the presence and absence of Ca2+, respectively, and was able to stimulate calmodulin-deficient cAMP phosphodiesterase. Based on its migration on SDS-PAGE gels, Mr and binding to anti-CaM antibodies it was deduced that calmodulin from P. nil is essentially identical to calmodulin isolated from other plants.
Similar content being viewed by others
References
Anderson, J.M., Charbonneau, H., Jones, H.P., McCann, R.O., Cormier, M.J.: Characterization of the plant nicotinamide adenine dinucleotide kinase activator protein and its identification as calmodulin.-Biochemistry 19: 3113-3120, 1980.
Bradford, M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.-Ann. Biochem. 72: 248-254, 1976.
Burgess, W.H., Jemiolo, D.K., Kretsinger, R.H.: Interaction of calcium and calmodulin in the presence of sodium dodecylsulfate.-Biochim. biophys. Acta 623: 257-270, 1980.
Campbell, K.P., MacLennan, D.H., Jorgensen, A.O.: Staining of the Ca2+-binding proteins, calsequestrin, calmodulin, troponin C, and S-100, with the cationic carbocyanine dye “Stains-all”.-J. biol. Chem. 258: 11267-11273, 1983.
Chafouleas, J.G., Dedman, J.R., Munjaal, R.P., Means, A.R.: Calmodulin. Development and application of a sensitive radioimmunoassay.-J. biol. Chem. 254: 10262-10267, 1979.
Dedmen, J.R., Means, A.R.: Characterization of a spectrophotometric assay for cAMP phosphodiesterase.-J. cyclic nucl. Acid Res. 3: 139-152, 1977.
Dubery, I.A., Schabort, J.C.: Calmodulin from Citrus saneness: purification and characterization.-Photochemistry 26: 37-40, 1987.
Friedman, H., Goldschmidt, E.E., Halevy, A.H.: Involvement of calcium in the photoperiodic flower induction of Pharbitis nil.-Plant Physiol. 89: 530-534, 1989.
Gong, M., Yang, Z.H., Tsao, T.H.: Isolation and characterization of calmodulin and a novel calcium-binding protein calpollenin from Pinus yunnanensis pollen.-Plant Sci. 89: 5-12, 1993.
Goplalakrishna, R., Anderson, W.B.: Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by Phenyl-Sepharose affinity chromatography.-Biochem. biophys. Res. Commun. 104: 830-836, 1982.
Jablonsky, P.P., Grolig, F., Perkin, J.L., Williamson, R.E.: Properties of monoclonal antibodies to plant calmodulin.-Plant Sci. 76: 175-184, 1991.
Jaworski, K., Szmidt-Jaworska, A., Tretyn, A., Kopcewicz, J.: Biochemical evidence for a calcium-dependent protein kinase from Pharbitis nil and its involvement in photoperiodic flower induction.-Phytochemistry 62: 1047-1055, 2003.
Kopcewicz, J., Tretyn, A.: Physiological and cytochemical investigation on photoperiodic floral induction in Pharbitis nil.-Flower. New Lett. 25: 26-34, 1998.
Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4.-Nature 227: 680-685, 1970.
Marmê, D., Dieter, P.: Role of Ca2+ in plants.-In: Cheung, W.Y. (ed.): Calcium and Cell Function. Pp. 263-311. Academic Press, New York 1983.
Millar, A.J., McGrath, R.B., Chua, N.H.: Phytochrome transduction pathways.-Annu. Rev. Genet. 28: 325-349, 1994.
Neuhaus, G., Bowler, C., Kern, R.: Calcium/calmodulin-dependent and-independent phytochrome signal transduction pathways.-Cell 73: 937-952, 1993.
Snedden, W. A., Form, H.: Calmodulin as a versatile calcium signal traducer in plants.-New Phytol. 151: 35-66, 2001.
Szmidt-Jaworska, A., Jaworski, K., Kesy, J., Kopcewicz, J.: Production and purification of polyclonal antibodies to Pisum and Avena labile phytochrome which cross-react with phyA from Pharbitis nil.-Acta Physiol. Plant. 22: 417-422, 2000.
Tretyn, A., Czaplewska, H., Cymerski, M., Kopcewicz, J., Kendsick, R.E.: The mechanism of calcium action on flower induction in Pharbitis nil.-J. Plant Physiol. 144: 562-568, 1994.
Tretyn, A., Czaplewska, H., Kopcewicz, J., Oleńczuk, A., Nowakowska, A.: The role of cotyledons in photoperiodic flower induction of Pharbitis nil.-In: Greppin, H., Panel, C., Simon, P. (ed.): Travelling Shot on Plant Development. Pp. 51-62, University of Geneva, Geneva 1997.
Van Eldik, L.J., Grossman, A.R., Iverson, D.B., Watterson, D.M.: Isolation and characterization of calmodulin from spinach leaves and in vitro translation mixture.-Proc. nat. Acad. Sci. USA 77: 1912-1916, 1980.
Watterson, D.M., Iverson, D.B., Van Eldik, L.J.: Spinach calmodulin: isolation, characterization, and comparison with vertebrate calmodulins.-Biochemistry 19: 5762-5768, 1980.
Zielinski, R.E.: Calmodulin and calmodulin-binding proteins in plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 49: 697-725, 1998.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Jaworski, K., Szmidt-Jaworska, A., Tretyn, A. et al. Calmodulin from Pharbitis nil: Purification and Characterization. Biologia Plantarum 48, 55–60 (2004). https://doi.org/10.1023/B:BIOP.0000024275.66196.d9
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000024275.66196.d9