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Activities of Soluble and Microsomal Farnesyl Diphosphatases in Datura stramonium

  • Published:
Biologia Plantarum

Abstract

Farnesyl diphosphatase (FDPase; EC 3.1.7.1) produces farnesol from farnesyl diphosphate (FDP) in a reaction that does not require Mg2+. This report shows that FDPase is constitutively expressed at a high level in the soluble and the microsomal fractions of Datura stramonium. Soluble and microsomal FDPase have a similar pH optimum (5.0 – 6.0) and a similar substrate specificity. Geranyl diphosphate (GDP) and geranylgeranyl diphosphate (GGDP) compete for FDP, but isopentenyl diphosphate (IDP), ATP, and para-nitrophenyl phosphate do not. Soluble FDPase activity was highest in fruit and flower followed by root, and leaf.

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Authors and Affiliations

  1. Department of Biotechnology, Agricultural Plant Stress Research Center, Chonnam National University, Gwangju, 500-757, South Korea

    S.B. Ha, D.E. Lee, H.J. Lee, S.J. Song & K. Back

Authors
  1. S.B. Ha
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  2. D.E. Lee
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  3. H.J. Lee
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  4. S.J. Song
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  5. K. Back
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Correspondence to K. Back.

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Ha, S., Lee, D., Lee, H. et al. Activities of Soluble and Microsomal Farnesyl Diphosphatases in Datura stramonium . Biologia Plantarum 47, 477–479 (2003). https://doi.org/10.1023/B:BIOP.0000023901.49409.7b

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  • DOI: https://doi.org/10.1023/B:BIOP.0000023901.49409.7b

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