Abstract
Rootstocks for sweet cherry (Prunus canescens Bois) Camil GM 79 were grown in vitro on Murashige and Skoog (MS) medium, and on MS medium with double- and half-strength macroelements. All the media contained 4.4 μM 6-benzyladenine, 0.5 μM α-naphthylacetic acid, 0.3 μM gibberellic acid, 20 g dm−3 sucrose and 7 g dm−3 agar. The chemical analyses were monitored on day 0 and 40 of culturing in callus, stem and leaves. Fresh and dry mass of shoots increased linearly up to the end of culture. The highest fresh and dry masses and also the content of Ca and Mg were registered in shoots grown on half-strength MS medium.
References
ĆaloviĆ, M.: Specifičnost Usvajanja Mineralnih Elemenata u Kalusnom Tkivu Kultivara Psenice (Triticum aestivum L.). [Specificity of the Mineral Elements Uptake in the Callus Tissue of Wheat (Triticum aestivum L.) Cultivars.]-Master's Thesis, Univ. Belgrade, Belgrade 1997. [In Serb.]
Mezei, S., SariĆ, M., PajeviĆ, S., Kovačev, L.: Concentration of mineral elements in callus tissue cultures of two sugar beet (Beta vulgaris L.) cultivars.-Arch. biol. Sci. 47: 29–32, 1995.
Mezzetti, B., Rosati, P., Casalicchio, G.: Actinidia deliciosa C.F. Liang in vitro. 1. Growth and mineral uptake by explants.-Plant Cell Tissue Organ Cult. 25: 91–98, 1991.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco cultures.-Physiol. Plant. 15: 473–497, 1962.
Orlikowska, T.: Effect of mineral composition and acidity of media, saccharose level, brand and quantity of agar on rooting of fruit rootstocks in vitro.-Biol. Plant. 34: 45–52, 1992.
RuziĆ, D.: Reakcija Nekih Genotipova Roda Prunus u Kulturi in vitro na Mineralni Sastav Medijuma. [The Reaction of Some Prunus Genotypes to Mineral Composition of the Medium in in Vitro Culture.]-Doctoral Thesis, Univ. Belgrade, Belgrade 1998. [In Serb.]
Singha, S., Oberly, G.H., Townsend, E.C.: Changes in nutrient composition and pH of the culture medium during in vitro shoot proliferation of crabapple and pear.-Plant Cell Tissue Organ Cult. 11: 209–220, 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ružić, Đ., Sarić, M., Cerović, R. et al. Contents of Macroelements and Growth of Sweet Cherry Rootstock in vitro . Biologia Plantarum 47, 463–465 (2003). https://doi.org/10.1023/B:BIOP.0000023897.84367.41
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000023897.84367.41