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Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum

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Biologia Plantarum

Abstract

The report describes in vitro plant regeneration from embryo axis explants of six cultivars of cotton. Induction of a maximum number of multiple shoots in all six cultivars could be achieved on Murashige and Skoog's (MS) salts and Gamborg's (B5) vitamins supplemented with 0.4 μM benzyladenine (BA) and 0.1 μM napthaleneacetic acid (NAA). Elongated shoots could be rooted on half strength medium supplemented with 0.5 μM NAA. Rooted shoots survived (92 %) after hardening in the greenhouse and grew to maturity (100 %) after transfer to field.

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Correspondence to D.C. Agrawal.

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Banerjee, A., Agrawal, D., Nalawade, S. et al. Multiple Shoot Induction and Plant Regeneration from Embryo Axes of Six Cultivars of Gossypium hirsutum . Biologia Plantarum 47, 433–436 (2003). https://doi.org/10.1023/B:BIOP.0000023889.20631.92

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  • DOI: https://doi.org/10.1023/B:BIOP.0000023889.20631.92

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