Abstract
Explants of four F1 hybrids (OMR 36-41/1, OMR 36-41/2, OMR 36-41/4 and OMR 36-41/5) and two cultivars (Rayong 1 and Rayong 60) of cassava (Manihot esculenta Crantz) were subjected to different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), kinetin (KIN) and N6-benzylaminopurine (BAP) to induce somatic embryogenesis, organogenesis and micropropagation. Shoot apices of the F1 hybrids exhibited higher frequency (62 – 74 %) of proliferation of somatic embryos than the cultivars (21 – 43 %) in Murashige and Skoog basal medium supplemented with 8 mg dm−3 2,4-D and 0.5 mg dm−3 NAA. Nodal explants of regenerated plantlets were rapidly micropropagated with 90 % efficiency on a medium containing 0.1 mg dm−3 NAA and 0.05 mg dm−3 BAP irrespective of explant source.
This is a preview of subscription content, log in via an institution to check access.
References
Byrne, D.H.: Breeding cassava.-In: Jannick, J. (ed.): Plant Breeding Reviews. Vol. 2. Pp. 73–134. AVI, Westport 1984.
Cock, J.H.: Cassava: New Potential for a Neglected Crop.-Wetview Press, Boulder-London 1985.
Das, G., Rout, G.R.: Plantlet regeneration through somatic embryogenesis in leaf derived callus of Plumago rosea.-Biol. Plant. 45: 299–302, 2002.
Kawano, K., Limsila, A., Tongglum, A., Suparhan, D.: Cassava cultivar evolution viewed through harvest index and biomass production.-In: Proc. 8th Int. Trop. Root Crops. Pp. 202–210. ISTRC, Bangkok 1990.
Konan, K.K., Sangwan, R.S., Sangnan-Norrel, B.S.: Efficient in vitro shoot-regeneration systems in cassava (Manihot esculenta Crantz).-Plant Breed 113: 227–236, 1994.
Liu, M.C.: The in vitro induction of callus and regeneration of cassava plants from shoot apical meristems.-Taiwan Sugar 22: 171–177, 1975.
Mathews, H., Schopke, C., Carcamo, R., Chavarriaga, P., Fauquet, C., Beachy, R.N.: Improvement of somatic embryogenesis and plant recovery in cassava.-Plant Cell Rep. 12: 328–333, 1993.
Mize, C.W., Chun, Y.W.: Analyzing treatment means in plant tissue culture research.-Plant Cell Tissue Organ Cult. 13: 201–217, 1988.
Mohan, M.L., Krishnamurthy, K.V.: Somatic embryogenesis and plant regeneration in pigeonpea.-Biol. Plant. 45: 19–25, 2002.
Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassay with tobacco tissue cultures.-Physiol. Plant. 15: 473–497, 1962.
Raemakers, C.J.J.M., Bessembinder, J.J.E., Staristky, G., Jacobsen, E., Visser, R.G.F.: Induction, germination and shoot development of somatic. embryos in cassava.-Plant Cell Tissue Organ Cult. 33: 151–156, 1993.
Smith, M.K., Biggs, B.J., Scott, K.J.: In vitro propagation of cassava (Manihot esculenta Crantz).-Plant Cell Tissue Organ Cult. 6: 221–228, 1986.
Stamp, J.A.: Somatic embryogenesis in cassava: the anatomy and morphology of the regeneration process.-Ann. Bot. 59: 451–459, 1987.
Stamp, J.A., Henshaw, G.G.: Somatic embryogenesis in cassava.-Z. Pflanzenphysiol. 105: 183–187, 1982.
Stamp, J.A., Henshaw, G.G.: Adventitious regeneration in cassava.-In: Withers, L.A., Andreson, P.G. (ed.): Plant Tissue Culture and its Agricultural Applications. Pp. 149–157. Butterworths, London 1986.
Stamp, J.A., Henshaw, G.G.: Somatic embryogenesis from clonal leaf tissue of cassava.-Ann. Bot. 59: 445–450, 1987a.
Stamp, J.A., Henshaw, G.G.: Secondary somatic embryogenesis and plant regeneration in cassava.-Plant Cell Tissue Organ Cult. 10: 227–233, 1987b.
Szabados, L., Hoyos R., Roca, W.: In vitro somatic embryogenesis and plant regeneration of cassava.-Plant Cell Rep. 6: 248–251, 1987.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ogburia, M. Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F1 Hybrids of Manihot esculenta . Biologia Plantarum 47, 429–432 (2003). https://doi.org/10.1023/B:BIOP.0000023888.82993.b1
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000023888.82993.b1