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Simultaneous Regeneration of Different Morphogenic Structures from Quince Leaves as Affected by Growth Regulator Combination and Treatment Length

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Biologia Plantarum

Abstract

Experiments were performed to evaluate the capacity of quince (Cydonia oblonga Mill.) leaves to regenerate somatic embryos and shoots and/or roots simultaneously. Leaves, treated for 2 d in liquid medium containing 2.5 mg dm−3 2,4-dichlorophenoxyacetic acid were cultured for 0, 3, 6, 9, 12, 15, 18, 21 d on a gelled medium supplemented with 1 mg dm−3 kinetin (Kin) and 0.1 mg dm−3 naphthalenacetic acid (NAA) and were transferred to a medium either without growth regulator (GR-) or containing 0.6 mg dm−3 6-benzylaminopurine (BA) + 0.2 mg dm−3 gibberellic acid (GA3) + 0.06 mg dm−3 indole-3-butyric acid (IBA) (GR+). Leaves producing somatic embryos (SEs) only, or adventitious roots (Rs) only, or SEs+Rs simultaneously, were detected on GR- culture medium; on GR+ medium, leaves producing adventitious shoots (Ss) only, SEs+Ss or SEs+Rs+Ss simultaneously, also appeared. Leaves producing both Ss+Rs were never detected. Proportions among the various types of regenerating leaves varied according to both the length of Kin+NAA treatment and the presence or absence of GR in the transfer medium. The greatest variations, both on GR− and on GR+, took place within the first 9 d of culturing on Kin+NAA. After this period, no further substantial differences in the trend of each type of regenerating leaf were observed. The length of the treatment with Kin+NAA also modified the proportions between the different types of morphogenic structures.

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Authors and Affiliations

  1. Dipartimento di Coltivazione e Difesa delle Specie Legnose “G. Scaramuzzi”, Sezione Coltivazioni Arboree, Università di Pisa, Via del Borghetto 80, I-56124, Pisa, Italy

    C. D'Onofrio & S. Morini

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  1. C. D'Onofrio
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  2. S. Morini
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Correspondence to S. Morini.

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D'Onofrio, C., Morini, S. Simultaneous Regeneration of Different Morphogenic Structures from Quince Leaves as Affected by Growth Regulator Combination and Treatment Length. Biologia Plantarum 47, 321–325 (2003). https://doi.org/10.1023/B:BIOP.0000023872.61646.95

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  • DOI: https://doi.org/10.1023/B:BIOP.0000023872.61646.95

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