Abstract
In four experiments, the chromosome doubling effect of acenaphthene vapour was tested on androgenic embryoids and/or plantlets of tobacco. The maximum rate of plants with doubled chromosome numbers (41.3 % diploids plus 2.7 % tetraploids) was recorded when approx. 100 mg acenaphthene in small and sterilized cheese-cloth bags were hanged for 72 h, into the culture jars (6.0 × 11.5 cm), containing 4 – 6 androgenic plantlets newly transferred from culture tubes. Acenaphthene treatments performed at the earlier stages of culture resulted in lower percentages (from 0.0 to 6.3 %).
This is a preview of subscription content, log in via an institution to check access.
References
Bürün, B., Emiroğlu, Ü.: [The effect of sucrose concentrations on the production of androgenetic haploids in tobacco.]-J. Fac. Agr. Ege Univ. 22(2): 29-42, 1985. [In Turkish.]
Collins, G.B., Sadasivaiah, R.S.: Meiotic analysis of haploid and doubled haploid forms of Nicotiana otophora and N. tabacum.-Chromosoma 38: 387-404, 1972.
Dai, M., Lu, H.Y., Shao, X.M.: Studies on chromosome doubling in embryoids derived from pollen in Nicotiana tabacum L.-Acta agron. sin. 10(1): 19-23, 1984.
Emiroğlu, Ü.: [Production of haploid plants from main Turkish tobacco varieties (Nicotiana tabacum L.) and meiosis and karyotype analyses on these haploids.]-Assoc. Prof. Thesis, Faculty of Agriculture, Ege Univ., Bornova-İzmir, 1974. [In Turkish.]
Emiroğlu, Ü., Sekin, S., Bürün, B.: [Breeding new tobacco lines for Aegean region through anther culture.]-Turk. J. Agr. Forest. 11: 334-347, 1987. [In Turkish.]
Goodspeed, T.H.: The Genus Nicotiana.-Chronica Botanica Company, Waltham 1954.
Kasperbauer, M.J., Collins, G.B.: Reconstitution of diploids from leaf tissue of anther-derived haploids in tobacco.-Crop Sci. 12: 98-101, 1972.
Kasperbauer, M.J., Wilson, H.M.: Haploid plant production and use.-In: Durbin, R.D. (ed.): Nicotiana Procedures for Experimental Use. Pp. 33-39. Univ. Wisconsin, Madison 1979.
Kefi, S.: [A study on the production of tobacco dihaploids by acenaphthene treatment.]-MSc Thesis, Ege Univ., Bornova-İzmir 1985. [In Turkish.]
Nitsch, J.P., Nitsch, C.: Haploid plant from pollen grains.-Science 163: 85-87, 1969.
Schiltz, P., Hitier, G., Cazamajour, F.: Utilization de l'androgenèse au cours de la sélection de Nicotiana tabacum.-Ann. Tabac 12(2): 11-17, 1975.
Smith, H.H.: The genus as a genetic resource.-In: Durbin, R.D. (ed.): Nicotiana Procedures for Experimental Use. Pp. 1-16. Univ. Wisconsin, Madison 1979.
Takashima, H., Hasegawa, H., Nakamura, A.: A simple method for chromosome doubling in tobacco anther culture.-Breed. Sci. 45: 107-110, 1995.
Walther, F.: Eine neue cytologische Untersuchungsmethode für Beta-Rüben.-Zucker 14: 274-276, 1961.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Bürün, B., EmİroĞlu, Ü. Production of Doubled Haploids from Androgenic Embryoids and Plantlets of Tobacco. Biologia Plantarum 47, 293–295 (2003). https://doi.org/10.1023/B:BIOP.0000022269.30902.5c
Issue Date:
DOI: https://doi.org/10.1023/B:BIOP.0000022269.30902.5c