Humans are frequently exposed to combinations of lead (Pb), cadmium (Cd) and Arsenic (As) but there is a paucity of actual data on the molecular effects of these agents at low dose levels. The present factorial design studies were undertaken in rats to examine the effects of these agents at LOEL dose levels on a number of molecular parameters of oxidative stress in hematopoietic and renal organ systems following oral exposure in drinking water at 30, 90 and 180 day time points. Results of these studies demonstrated dynamic, time-dependent alterations in both molecular targets and inducible oxidative stress protective systems in target cell populations. In general, cellular protective systems, which protected against oxidative damage at the 90 day time point, appeared to be finite such that molecular manifestations of oxidative stress became statistically significant at the 180 day time point for several of the combination exposure groups. These data demonstrate the importance of duration of exposure in assessing the toxic potential of Pb, Cd and As mixtures at low dose levels.
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Fowler BA, Mahaffey KR. 1978 Interaction between lead, cadmium and arsenic in relation to porphyrin excretion patterns. Environ Hlth Perspec 25, 87–90.
Madden EF, Fowler BA. 2000 Mechanisms of nephrotoxicity from metal combinations: A review. Drug Chem Toxicol 23, 1–12.
Madden EF, Akkerman M, Fowler BA. 2002 A comparison of 60 kDA, 70 kDA and 90 kDA stress protein expression in nor-mal rat NRK-52 and human HK-2 kidney cell lines following in vitro exposure to arsenite and cadmium alone or in combination. J Biochem Mol Toxicol 16, 24–32.
Mahaffey KR, Capar SG, Gladen BC, Fowler BA. 1981 Concurrent exposure to lead cadmium and arsenic: effects on toxicity and tissue metal concentrations in the rat. J Lab Clin Med 98, 463–481.
Wang G, Chen X-Q, Lipsky MM, Whittaker MH, Fowler BA. 2004 (in press), Interactions of lead, cadmium and arsenic in rat kidneys. The Toxicologist(Abstract).
Whittaker MH, Lipsky MM, Wang G, Chen X-Q, Fowler BA. 2004 (in press) Oxidative stress induced by lead, cadmium and ar-senic mixtures: 180-day drinking water studies in rats. The Toxicologist (Abstract).
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Fowler, B.A., Whittaker, M.H., Lipsky, M. et al. Oxidative stress induced by lead, cadmium and arsenic mixtures: 30-day, 90-day, and 180-day drinking water studies in rats: An overview. Biometals 17, 567–568 (2004). https://doi.org/10.1023/B:BIOM.0000045740.52182.9d
- oxidative stress