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Monitoring of cobalt(II) uptake and transformation in cells of the plant-associated soil bacterium Azospirillum brasilense using emission Mössbauer spectroscopy

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Abstract

Interaction of cobalt(II) at micromolar concentrations with live cells of the plant-growth-promoting rhizobacterium Azospirillum brasilense (strain Sp245) and further transformations of the metal cation were monitored using 57Co emission Mössbauer spectroscopy (EMS). Cell suspensions of the bacterial culture (2.4×108 cells ml−1) were doped with radioactive 57CoCl2 (1 mCi; final concentration 2×10−6 M 57Co2+), kept under physiological conditions for various periods of time (from 2 min up to 1 hour) and then rapidly frozen in liquid nitrogen. Analysis of emission Mössbauer spectra of the frozen aqueous suspensions of the bacterial cell samples shows that the primary absorption of cobalt(II) at micromolar concentrations by the bacterial cells is rapid and virtually complete, giving at least two major forms of cobalt(II) species bound to the cells. Within an hour, the metal is involved in further metabolic transformations reflected by changes occurring in the spectra. The Mössbauer parameters calculated from the EMS data by statistical treatment were different for suspensions of live and dead (thermally killed) bacterial cells that had been in contact with 57Co2+ for 1 h, as well as for the cell-free culture medium containing the same concentration of 57Co2+. Chemical after-effects of the nuclear transition (57Co →57Fe), which provide additional information on the chemical environment of metal ions, are also considered. The data presented demonstrate that EMS is a valuable tool for monitoring the chemical state of cobalt species in biological matter providing information at the atomic level in the course of its uptake and/or metabolic transformations.

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Authors and Affiliations

  1. Laboratory of Biochemistry of Plant-Bacterial Symbioses, Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, 410049, Saratov, Russia

    Alexander A. Kamnev & Lyudmila P. Antonyuk

  2. Laboratory of Nuclear Chemistry Techniques, Department of Radiochemistry, Faculty of Chemistry, Moscow State University, 11991, Moscow, Russia

    Leonid A. Kulikov & Yury D. Perfiliev

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  1. Alexander A. Kamnev
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  2. Lyudmila P. Antonyuk
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  3. Leonid A. Kulikov
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  4. Yury D. Perfiliev
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Correspondence to Alexander A. Kamnev.

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Kamnev, A.A., Antonyuk, L.P., Kulikov, L.A. et al. Monitoring of cobalt(II) uptake and transformation in cells of the plant-associated soil bacterium Azospirillum brasilense using emission Mössbauer spectroscopy. Biometals 17, 457–466 (2004). https://doi.org/10.1023/B:BIOM.0000029442.72234.2e

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