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Lanthanide ions promote the hydrolysis of 2,3-Bisphosphoglycerate

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Abstract

The 31P NMR studies showed that lanthanide ions promote the site-specific hydrolysis of 2,3-Bisphosphoglycerate (BPG) at pH 7.4 by cleaving the 2′ phosphomonoester bond. The effect of fourteen trivalent lanthanide ions and Sc3+, and Y3+ were compared by the percentage of hydrolysis obtained by determining the inorganic phosphate produced. All the trivalent lanthanide ions promote the hydrolysis, but Sc3+ not. Among them, Ce3+ affects the reaction mostly. This was mainly attributed to the autooxidation of Ce3+ to Ce4+, since the promoting effect of Ce3+ is related to the increasing Ce4+ amount in the solution and depressed by adding sulphite. Ce4+ promotes the hydrolysis more efficiently than Ce3+ do. The pseudo first-order rate constant for the hydrolysis of BPG by Ce(SO4)2 (18.7 mM) at pH 1 and pH 2, 37 °C is 3.1 h−1 and 0.65 h−1 respectively. A mechanism with a hydroxo species as reactive intermediate was proposed for the trivalent lanthanide ions. The site-specificity was explainable by this mechanism.

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Authors and Affiliations

  1. INSERM U119, Institut Paoli-Calmettes, 27 bd Lei Roure, 13009, Marseille, France

    Bing Zhu

  2. Department of Chemical Biology, School of Pharmaceutical Sciences, Peking University, Beijing, 100083, China

    Deping Xue & Kui Wang

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  1. Bing Zhu
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  2. Deping Xue
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  3. Kui Wang
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Correspondence to Bing Zhu.

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Zhu, B., Xue, D. & Wang, K. Lanthanide ions promote the hydrolysis of 2,3-Bisphosphoglycerate. Biometals 17, 423–433 (2004). https://doi.org/10.1023/B:BIOM.0000029439.05958.d9

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  • DOI: https://doi.org/10.1023/B:BIOM.0000029439.05958.d9

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