Abstract
Transmural distribution of low molecular weight iron (LMWI), total iron, and protein carbonyls (PC) was investigated in the perfused rabbit heart under aerobic conditions, and after 60 min hypoxia followed or not by 3 min reoxygenation. In the aerobic perfused hearts, LMWI, total iron and PC did not show significant transmural differences. Hypoxia increased LMWI and PC levels, which were significantly higher in the subendocardium than in the subepicardium; further significant changes were not observed after reoxygenation. Total iron showed no transmural difference and was not significantly affected by both hypoxia and reoxygenation. Free iron was undetectable in the myocardial effluent of all experimental groups. Thus, hypoxia favors myocardial iron decompartmentalisation and oxidative stress, which are significantly greater in the inner than in the outer ventricular layers. Such findings may add further insight into the problem of the vulnerability of the mammalian subendocardium to injury induced by oxygen deprivation.
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References
Artiss JD, Vinogradov S, Zak B. 1981 Spectrophotometric study of several sensitive reagents for serum iron. Clin Biochem 14, 311-315.
Bradford MM. 1976 A rapid and sensitive method for the quantitation of microgram quantities of protein using the principle of protein-dye binding. Anal Biochem 72, 248-254.
Braunwald E, Kloner RA. 1985 Myocardial reperfusion: a double-edged sword? J Clin Invest 76, 1713-1719.
Brumby PE, Massey V. 1967 Determination of nonheme iron, total iron, and copper. Methods Enzymol 10, 463-474.
Dhaliwal H, Kirshenbaum LA, Randhawa AK, Singal PK. 1991 Correlation between antioxidant changes during hypoxia and recovery on reoxygenation. Am J Physiol 261, H632-H638.
Doeg KA, Ziegler DM. 1962 Simplified methods for the estimation of iron in mitochondria and submitochondrial fractions. Arch Biochem Biophys 97, 37-40.
Funk F, Lenders J-P, Crichton RR, Schneider W. 1985 Reductive mobilisation of ferritin iron. Eur J Biochem 152, 167-172.
Glantz SA. 1987 Primer of Biostatistics. New York: McGraw-Hill.
Grabellus F, Schmid C, Levkau B, Breukelmann D, Halloran PF, August C, Takeda N, Takeda A, Wilhelm M, Deng MC, Baba HA. 2002 Reduction of hypoxia-inducible heme oxygenase-1 in the myocardium after left ventricular mechanical support. J Pathol 197, 230-237.
Halliwell B, Gutteridge JMC. 1990 Role of free radicals and catalytic metal ions in human disease: An overview. Methods Enzymol 186, 1-85.
Healing G, Gower J, Fuller B, Green C. 1990 Intracellular iron redistribution. An important determinant of reperfusion damage to rabbit kidney. Biochem Pharmacol 39, 1239-1245.
Hoshida S, Kuzuya T, Nishida M, Kim Y, Fuji H, Kamada T, Tada M. 1988 Regional free radical generation in canine reperfused ischemic myocardium. Circulation 77(Suppl. II), II-373 (abstr)
Humphrey SM, Gavin JB. 1984 The effect of coronary pressure on contracture and vascular perfusion in the hypoxic isolated rat heart. Basic Res Cardiol 79, 350-362.
Lapenna D, Mezzetti A, de Gioia S, Consoli A, Festi D, Di Ilio C, Cuccurullo F. 1994 Transmural distribution of antioxidant defences and lipid peroxidation in the rabbit left ventricular myocardium. Pflügers Archiv (Eur J Physiol) 427, 432-436.
Lapenna D, Porreca E, Mezzetti A, de Gioia S, Marzio L, Cuccurullo F. 1993 Hypoxia-induced coronary flow changes in the perfused rat heart: Effects of high L-carnitine concentrations. Gen Pharmac 24, 211-215.
Minamidate AS, Takano T, Hashikawa T, Abiko Y. 1973 Transmuaral gradient of NAD+/NADH ratio in the canine left ventricular myocardium and effects of coronary dilators on the transmural gradient. Jpn J Pharmacol 23, 126-128.
Mulligan M, Althaus B, Linder MC. 1986 Non-ferritin, non-heme iron pools in rat tissues. Int J Biochem 18, 791-798.
Nohl H, Koltover V, Stolze K. 1993 Ischemia/reperfusion impairs mitochondrial energy conservation and triggers O -2 release as a byproduct of respiration. Free Rad Res Commun 18, 127-137.
Oliver CN, Ahn B, Moerman EJ, Goldstein S, Stadtman ER. 1987 Age-related changes in oxidized proteins. J Biol Chem 262, 5488-5491.
Park Y, Kanekal S, Kehrer JP. 1991 Oxidative changes in hypoxic rat heart tissue. Am J Physiol 260, H1395-H1405.
Reif DW. 1992 Ferritin as a source of iron for oxidative damage. Free Radic Biol Med 12, 417-427.
Reimer KA, Jennings RB, Tatum AH. 1983 Pathobiology of acute myocardial ischemia: Metabolic, functional and ultrastructural studies. Am J Cardiol 52, 72A-81A.
Suttner DM, Dennery PA. 1999 Reversal of HO-1 related cytoprotection with increased expression is due to reactive iron. FASEB J 13, 1800-1808.
van Jaarsveld H, Kuyl JM, De Wet EH, Alberts DW, van der Westhuizen FD. 1991 Effect of various mixtures of diethylether, halotane, nitrous oxide and oxygen on low molecular weight iron content and mitochondrial function of the rat myocardium. Free Radic Res Commun 15, 151-157.
Voogd A, Sluiter W, van Eijk HG, Koster JF. 1992 Low molecular weight iron and the oxygen paradox in isolated rat hearts. J Clin Invest 90, 2050-2055.
Watson RM, Markle DR, Ro YM, Goldstein SR, McGuire DA, Peterson JI, Patterson RE. 1984 Transmural pH gradient in canine myocardial ischemia. Am J Physiol 246, H232-H238.
Wolfgang GHI, Jolly RA, Petry TW. 1991 Diquat-induced oxidative damage in hepatic microsomes. Effects of antioxidants. Free Radic Biol Med 10, 403-411.
Zweyer JL, Kuppusamy P, Williams R, Rayburn BK, Smith D, Weisfeldt ML, Flaherty JT. 1989 Measurement and characterization of posthischemic free radical generation in the isolated perfused heart. J Biol Chem 264, 18890-18895.
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Lapenna, D., Ciofani, G., Pierdomenico, S.D. et al. Transmural distribution of iron in the hypoxic and reoxygenated rabbit left ventricular myocardium. Biometals 17, 359–363 (2004). https://doi.org/10.1023/B:BIOM.0000029418.57096.f7
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DOI: https://doi.org/10.1023/B:BIOM.0000029418.57096.f7