Abstract
This paper provides the details of the Coupled Biological and Chemical (CBC)model for representing in situ bioremediation of BTEX. The CBC modelcontains novel features that allow it to comprehensively track the footprints ofBTEX bioremediation, even when the fate of those footprints is confounded byabiotic reactions and complex interactions among different kinds of microorganisms.To achieve this comprehensive tracking of all the footprints, the CBC modelcontains important new biological features and key abiotic reactions. The biologicalmodule of the CBC-model includes these important new aspects: (1) it separatesBTEX fermentation from methanogenesis, (2) it explicitly includes biomass as asink for electrons and carbon, (3) it has different growth rates for each biomasstype, and (4) it includes inhibition of the different reactions by other electronacceptors and by sulfide toxicants. The chemical module of the CBC-modelincludes abiotic reactions that affect the footprints of the biological reactions.In particular, the chemical module describes the precipitation/dissolution ofCaCO3, Fe2O3, FeS, FeS2, and S°. The kinetics for the precipitation/dissolution reactions follow the critical review in Maurer and Rittmann (2003a).
Similar content being viewed by others
References
Ballapragada BS, Stensel, HD, Puhakka JA & Ferguson JF (1997) Effect of hydrogen on reductive dechlorination of chlorinated ethenes. Environ. Sci. Technol. 31(6): 1728-1734
Bjerg PL, Jakobsen R, Bay H, Rasmussen M, Albrechtsen H-J & Christensen PH (1997) Effects of well sampling construction on H 2 measurements made for characterization of redox conditions in a contaminated aquifer. Environ. Sci. Technol. 31(10): 3029–3031
Bradley PM & Chapelle FH (1997) Kinetics of DCE and VC mineralization under methanogenic and Fe(III)-reducing conditions. Environ. Sci. Technol.31: 2692-2696
Bradley PM& Chapelle FH (1998a) Microbial mineralization of VC and DCE under different terminal electron accepting conditions. Anaerobe. 4: 81-87
Bradley PM & Chapelle FH (1998b) Effect of contaminant concentration on aerobic microbial mineralization of DCE and VC in stream-bed sediments. Environ. Sci. Technol. 32(5): 553-557.
Bradley PM, Chapelle FH & Lovley. DR (1998a) Humic acids as electron acceptors for anaerobic microbial oxidation of vinyl chloride and dichloroethene. Appl. Environ. Microbiol. 64(8): 3102-3105
Bradley PM, Landmeyer JE & Dinicola RS (1998b) Anaerobic oxidation of [1, 2-14 C]dichloroethene under Mn(IV)-reducing conditions. Appl. Environ. Microbiol. 64: 1560-1562
Bradley PM, Chapelle FH & Wilson JT (1997) Field and laboratory evidence of intrinsic biodegradation of vinyl chloride contamin-ation in a Fe(III)-reducing aquifer. J. Contam. Hydrol. 31(1998): 111-127
Bradley PM & Chapelle FH (1996) Anaerobic mineralization of vinyl chloride in Fe(III)-reducing aquifer sediments. Environ. Sci. Technol. 30: 2084-2086
Capiro NL, McDade JM, Adamson DT, Bedient PB & Hughes JB (2002) Hydraulic effects of hydrogen delivery for treatment of chlorinated DNAPL. Third International Conference on Remedi-ation of Chlorinated and Recalcitrant Compounds. 20-23 May 2002, Monterey, CA
Canfield DE, Thamdrup B & Hansen. JW (1993) Geochim. Cosmochim. Acta. 57: 3867-3883
Chapelle FH, Vroblesky DA, Woodward JC & Lovely DR (1997) Practical considerations for measuring hydrogen concentrations in groundwater. Environ. Sci. Technol. 31(10): 2873-2877
Chapelle FH (1996) Identifying redox conditions that favor the natural attenuation of chlorinated ethenes in contaminated ground water systems. Proceedings of the Symposium on Natural Attenuation of Chlorinated Organics in Ground Water, 11-13
Chapelle FH, McMahon PB, Dubrovsky, NM, Fugii RF, Oaks-ford ET & Vroblesky DA (1995) Deducing the distribution of terminal electron-accepting processes in hydrologically diverse groundwater systems. Water Resour. Res. 31: 359-371
Cozzarelli IM, Suflita JM, Ulrich GA, Harris SH, Scholl MA, Schlottmann JL & Christenson S (2000) Geochemical and microbial methods for evaluating anaerobic processes in an aquifer contaminated by landfill leachate. Environ. Sci. Technol. 34(18): 4025-4033
DiStefano TD, Gossett JM & Zinder SH (1992) Hydrogen as an electron donor for dechlorination of tetrachloroethene by an anaerobic mixed culture. Appl. Environ. Microbiol. 58(11): 3622-3629
Fennell DE & Gossett JM (1998) Modeling the production of and competition for hydrogen in a dechlorinating culture. Environ. Sci. Technol. 32: 2450-2460
Gieg LM, Kolhatkar RV, McInerney MJ, Tanner RS, Harris Jr SH, Sublette KL & Suflita JM (1999) Intrinsic bioremediation of petroleum hydrocarbons in a gas condensate-contaminated aquifer. Environ. Sci. Technol. 33(15): 2550-2560
Goodwin S, Conrad R & Zeikus 1988. Influence of pH on microbial hydrogen metabolism in diverse sedimentary ecosystems. Appl. Environ. Microbiol. 54(2): 590-593
Hartmans S. & de Bont JAM (1992) Aerobic vinyl chloride metabolism in Mycobacterium aurum l i. Appl. Environ. Microbiol. 54: 1220-1226
Hendrickson ER, Payne JA, Young RM, Starr MG, Perry MP, Fahnestock S, Ellis DE & Ebersole RC (2002) Molecular analysis of Dehalococcoides 16S ribosomal DNA from chloroethene-contaminated sites throughout North America and Europe. Appl. Environ. Microbiol. 68(2): 485-495
Holliger C, Hahn D, Harmsen H, Ludwig W, Schumacher B, Tindall F, Vazquez F, Weiss N & Zehnder AJB (1998) Dehalobacter restrictusgen. nov and sp. nov., a strictly anaerobic bacterium that reductively dechlorinates tetra and trichloroethene in an anaerobic respiration. Arch. Microbiol. 169: 313-321
Hydro-Search, Inc (1994) Remedial Investigation Report Refuse Hideaway Landfill, Middleton, WI, Volume I. Project Number 301483135
Jakobsen R, Albrechtsen H-J, Rasmussen M, Bay H, Bjerg P & Christensen TH (1998) H2 concentrations in a landfill leachate plume (Grindsted, Denmark): In situ energetics of terminal electron acceptor processes. 32(14): 2142-2148
Kampbell DH, Wilson JT & McInnes AM (1998) Determining dissolved hydrogen, methane, and vinyl chloride concentrations in aqueous solutions on a nanomolar scale with the bubble strip method. Proceedings of the 1998 Conference on Hazardous Waste Research (pp. 187-201)
Koenigsberg S, Sandefur C & Lapus K (2001) Time-release electron donor technology: Results of forty-two field applications. In Magar VS, Fennell DE, Alleman BC & Leeson A (eds) Anaerobic degradation of chlorinated solvents. Vol 6(7) (pp 257–264). The Sixth International In Situ and On-Site Bioremediation Symposium, San Diego, CA., 4-7 June 2001. Battelle Press, Columbus, OH
Lovely DR, Chapelle FH & Woodward JC (1994) Use of dissolved H 2 concentrations to determine distribution of microbi-ally catalyzed redox reactions in anoxic groundwater. Environ. Sci. Technol. 28(7): 1205-1210
Lovley DR & Goodwin S (1988) Hydrogen concentrations as an indicator of the predominant terminal electron-accepting reactions in aquatic sediments. Geochim. Cosmochim. Acta. 52: 2993-3003
Marsh TL & McInerney MJ (2001) Relationship of hydrogen bioavailability to chromate reduction in aquifer sediments. Appl. Environ. Microbiol. 67(4): 1517-1521
Maymo-Gattell X, Anguish T, Gossett JM & Zinder SH (1999) Reductive dechlorination of chlorinated ethenes and 1,2-dichloroethane by “Dehalococcoides ethenogenes” 195. Appl. Environ. Microbiol. 65: 3108-3113
Maymo-Gattell X, Chien Y, Gossett JM & Zinder SH (1997) Isolation of a bacterium that reductively dechlorinates tetrachloro-ethene to ethene. Science 276: 1568-1571
Maymo-Gatell X, Tandoi V, Gossett JM & Zinder SH (1995) Characterization of an H 2-utilizing enrichment culture that reductively dechlorinates tetrachloroethylene to vinyl chloride and ethene in the absence of methanogenesis and acetogenesis. Appl. Environ. Microbiol. 61: 3928-3933
McCarty PL & Semprini L (1994) Groundwater treatment for chlorinated solvents. In Norris RD et al. (eds) Handbook of bioremediation. Lewis Publishers, Boca Raton, FL
McInnes D & Kampbell DH (2000) The bubble strip method of determining dissolved hydrogen (H 2 ) in well water. Field Analytical Chemistry and Technology. 4(6): 283-296
Microseeps (2002) Microseeps gas stripping cell instructions. Pitts-burgh, PA. http://www.microseeps.com
Montgomery Watson (1997) Predesign and additional studies work plan: Refuse hideaway landfill, Middleton, WI. Project Number 1242161.01120201. Montgomery Watson, Madison, WI.
Mormile MR, Koteswara RG, Robinson JA, McInerney MJ & Suflita JM (1996) The importance of hydrogen in landfill fermentation. Appl. Environ. Microbiol. 62(5): 1583-1588
Newell CJ, Haas PE, Hughes JB & Khan T (2000) Results from two direct hydrogen delivery field tests for enhanced dechlorination. 2nd International Conference on Remediation of Chlorinated and Recalcitrant Compounds, 22-25 May, 2000, Monterey, CA
Scholz-Muramatsu H, Neumann A, Messmer M, Moore E & Diekert G (1995) Isolation and characterization of Dehalospirillum multivoransgen. nov., sp. nov., a tetrachloroethylene-utilizing, strictly anaerobic bacterium. Arch. Microbiol. 163: 48-56
Smatlak CR, Gossett JM and Zinder SH (1996) Comparative kin-etics of hydrogen utilization for reductive dechlorination of tetrachloroethene and methanogenesis in an anaerobic enrichment culture. Environ. Sci. Technol. 30: 2850-2858
U.S. EPA (1998) Technical protocol for evaluating natural atten-uation of chlorinated solvents in ground water. Office of Research and Development, EPA/600/R-98/128 September 1998, available at http://www.epa.gov/ada/pubs/reports.html
Vroblesky DA, Bradley PM & Chapelle FH (1996) Environ. Sci. Technol. 30: 1377-1381
Wiedemeier TH, Rifai HS, Newell CJ & Wilson JT (1999) Natural attenuation of fuels and chlorinated solvents in the subsurface. John Wiley & Sons, Inc. New York, NY
Wisconsin Department of Natural Resources (1995) Superfund fact sheet: Proposed plan refuse hideaway landfill. Town of Middleton, WI. Terry Evanson, Project Manager
Yang Y & McCarty PL (1998) Competition for hydrogen within a chlorinated solvent dehalogenating anaerobic mixed culture. Environ. Sci. Technol. 32: 3591-3597.
Rights and permissions
About this article
Cite this article
Maurer, M., Rittmann, B.E. Formulation of the CBC-Model for Modelling the Contaminants and Footprints in Natural Attenuation of BTEX. Biodegradation 15, 475–485 (2004). https://doi.org/10.1023/B:BIOD.0000044588.86054.05
Issue Date:
DOI: https://doi.org/10.1023/B:BIOD.0000044588.86054.05