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Effects of Fungal Bioaugmentation and Cyclodextrin Amendment on Fluorene Degradation in Soil Slurry

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Abstract

This study assesses the potential of fungal bioaugmentationand the effect of maltosyl-cyclodextrin amendment, as anapproach to accelerate fluorene biodegradation in soil slurries.47 fungal strains isolated from a contaminated site weretested in the biodegradation of fluorene. Results showed thegreater efficiency of ``adaptated'' fungi isolated fromcontaminated soil vs. reference strains belonging to thecollection of the laboratory. These assays allowed us toselect the most efficient strain, Absidia cylindrospora, whichwas used in a bioaugmentation process. In the presence ofAbsidia cylindrospora, more than 90% of the fluorene wasremoved in 288 h while 576 h were necessary in the absenceof fungal bioaugmentation. Maltosyl-cyclodextrin, abranched-cyclodextrin was chosen in order to optimize fluorenebioavailability and biodegradation in soil slurries. The resultsof this study indicate that Absidia cylindrospora andmaltosyl-cyclodextrin could be used successfully in bioremediation systems.

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Authors and Affiliations

  1. UMR LECA 5553, Equipe PEX, Bâtiment D de Biologie, Université J. Fourier, BP 53, 38041, Grenoble Cedex 09, France

    David Garon, Lucile Sage, Fran¸oise Seigle-Murandi & Fran¸oise Seigle-Murandi

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  1. David Garon
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  2. Lucile Sage
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  3. Fran¸oise Seigle-Murandi
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Correspondence to Fran¸oise Seigle-Murandi.

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Garon, D., Sage, L. & Seigle-Murandi, F. Effects of Fungal Bioaugmentation and Cyclodextrin Amendment on Fluorene Degradation in Soil Slurry. Biodegradation 15, 1–8 (2004). https://doi.org/10.1023/B:BIOD.0000009934.87627.91

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  • DOI: https://doi.org/10.1023/B:BIOD.0000009934.87627.91

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