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Enhancement of ajmalicine production in Catharanthus roseuscell cultures with methyl jasmonate is dependent on timing and dosage of elicitation

Biotechnology Letters volume 26, pages 1595–1599 (2004)Cite this article

Abstract

The optimum growth stage for enhancing ajmalicine production in Catharanthus roseuscultures with methyl jasmonate (MJ) was after 6 d growth. MJ added at 10 or 100 μm on day 6 gave a maximum ajmalicine production of 10.2 mg l−1, a 300% increase over that of non-elicited cultures.

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References

  • Aerts RJ, Gisi D, De Carolis E, De Luca V, Baumann TW (1994) Methyl jasmonate vapor increases the developmentally con-trolled synthesis of alkaloids in Catharanthus and Cinchona seedlings. Plant J. 5: 635–43.

  • Asada M, Shuler ML (1989) Stimulation of ajmalicine production and excretion from Catharanthus roseus: effects of adsorption in situ, elicitors, and alginate immobilization. Appl. Microbiol. Biotechnol. 30: 475–481.

    Google Scholar 

  • Collu G, Unver N, Peltenburg-Looman AMG, van der Heijden R, Verpoorte R, Memelink J (2001) Geraniol 10-hydroxylase, a cytochrome P450 enzyme involved in terpenoid indole alkaloid biosynthesis. FEBS Lett. 508: 215–220.

    Google Scholar 

  • Contin A, van der Heijden R, ten Hoopen HJG, Verpoorte R (1998) The inoculum size triggers tryptamine or secologanin biosyn-thesis in a Catharanthus roseus cell culture. Plant Sci. 139: 205–211.

    Google Scholar 

  • Gantet P, Imbault N, Thiersault M, Doireau P (1998) Necessity of a functional octadecanoic pathway for indole alkaloid synthesis by Catharanthus roseus cell suspensions cultured in an auxin-starved medium. Plant Cell Physiol. 39: 220–225.

    Google Scholar 

  • Gundlach H, Muller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc. Natl. Acad. Sci. USA 89: 2389–2393.

    Google Scholar 

  • Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol. Bioeng. 62: 97–105.

    Google Scholar 

  • Lee-Parsons CWT, Shuler ML (2002) The effect of ajmalicine spiking and resin addition timing on the production of indole alkaloids from Catharanthus roseus cell cultures. Biotechnol. Bioeng. 79: 408–415.

    Google Scholar 

  • Mizukami H, Tabira Y, Ellis BE (1993) Methyl jasmonate-induced rosmarinic acid biosynthesis in Lithospermum erythrorhizon cell suspension cultures. Plant Cell Rep. 12: 706–709.

    Google Scholar 

  • Qian Z-G, Zhao Z-J, Xu Y, Qian X, Zhong J-J (2004) Novel chemically synthesized hydroxyl-containing jasmonates as powerful inducing signals for plant secondary metabolism. Biotechnol. Bioeng. 86: 809–816.

    Google Scholar 

  • Rijhwani, SK, Shanks JV (1998) Effect of elicitor dosage and exposure time on biosynthesis of indole alkaloids by Catharanthus roseus hairy root cultures. Biotechnol. Progr. 14: 442–449.

    Google Scholar 

  • van der Fits L, Memelink J (2000) ORCA3, a jasmonate-responsive transcriptional regulator of plant primary and secondary metabolism. Science 289: 295–297.

    Google Scholar 

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Authors and Affiliations

  1. Chemical Engineering Department, Northeastern University, 342 Snell Engineering Center, 360 Huntington Avenue, Boston, MA, 02115-5000, USA

    Carolyn W.T. Lee-Parsons & Seda Ertürk

  2. Bioengineering, Genzyme, Corp., 45 New York Ave., Framingham, MA, 01701, USA

    Jennifer Tengtrakool

Authors
  1. Carolyn W.T. Lee-Parsons
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  2. Seda Ertürk
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  3. Jennifer Tengtrakool
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Lee-Parsons, C.W., Ertürk, S. & Tengtrakool, J. Enhancement of ajmalicine production in Catharanthus roseuscell cultures with methyl jasmonate is dependent on timing and dosage of elicitation. Biotechnology Letters 26, 1595–1599 (2004). https://doi.org/10.1023/B:BILE.0000045825.37395.94

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  • DOI: https://doi.org/10.1023/B:BILE.0000045825.37395.94

  • ajmalicine
  • alkaloid
  • Catharanthus roseus
  • elicitation
  • methyl jasmonate