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The effect of soluble LAG-3 (CD223) treatment in fetal thymic organ culture

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Abstract

Lymphocyte activation gene-3 (LAG-3; CD223) is structurally similar to CD4 and binds to MHC class II with a 100-fold higher affinity than that of CD4. Soluble LAG-3 (sLAG-3Ig) might be useful for immunotherapy by inducing MHC class II-mediated cell activation. A new form of sLAG-3Ig was constructed containing a critical binding site (D1 and D2 region) to MHC class II, combined with a Fc portion of an immunoglobulin γ1. After treatment of sLAG-3Ig in fetal thymic organ culture from DO11.10 transgenic mouse, CD4+ T cell precursors were increased in the positive selection but not affected in the negative selection. Further analysis by treating sLAG-3Ig on thymic epithelial cells revealed that CD40 and MHC class II were up-regulated. These results may demonstrate that the treatment of sLAG-3Ig increases the precursor frequency of CD4+ T cells by activation of thymic epithelial cells.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, School of Medicine, Hanyang University, Haeng-Dang Dong, Sung-Dong Ku, Seoul, 133-791, South Korea

    Taehoon Chun, Hyun-Jung Byun, Hee Yong Chung & Yong-Hoon Chung

  2. Biomedical Research Institute MedExGen Inc., Medical Building #1, Haeng-Dang Dong, Sung-Dong Ku, Seoul, 133-791, South Korea

    Yong-Hoon Chung

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  1. Taehoon Chun
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  2. Hyun-Jung Byun
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  3. Hee Yong Chung
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  4. Yong-Hoon Chung
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Chun, T., Byun, HJ., Chung, H.Y. et al. The effect of soluble LAG-3 (CD223) treatment in fetal thymic organ culture. Biotechnology Letters 26, 1371–1377 (2004). https://doi.org/10.1023/B:BILE.0000045635.63873.96

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  • DOI: https://doi.org/10.1023/B:BILE.0000045635.63873.96

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