Skip to main content
SpringerLink
Log in

A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A novel tetrafunctional reagent, α,γ,α′,γ′-tetra-succinimidyl-hexanediamide-di-glutamate ester (HDG(OSu)4), was successfully synthesized, and a well-defined cross-linked bovine hemoglobin (mainly 128 kDa) was prepared with this reagent. Due to the spatial structure of this cross-linking reagent, the intramolecular and intermolecular cross-linking of bovine hemoglobin was formed simultaneously in one reaction. Although the cross-linked bovine hemoglobin showed a slight decrease in half-saturated O2 pressure value (P50, from 28.1 mm Hg to 21.7 mm Hg) and Hill coefficient (from 2.5 to 2), due to the cross-linkage, it still performed well for O2 delivery.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chang TMS (1999) Future prospect for artificial blood. Trends Biotechnol. 181: 61-67.

    Google Scholar 

  • Cheung LC, Storm CB, Gabriel BW, Anderson WA (1984) The preparation of stroma-free hemoglobin by selective DEAE-Cellulose absorption. Anal. Biochem. 137: 481-484.

    Google Scholar 

  • Keipert PE, Chang TMS (1984) Preparation and in vitro characteristics of a blood substitute based on pyridoxylated polyhemoglobin. Appl. Biochem. Biotechnol. 10: 133-141.

    Google Scholar 

  • Laemmli UK (1970) Cleavage of structural protein during the assembly of the head of bacteriophage T4. Nature 227: 680-685.

    Google Scholar 

  • Nelson D, Hai T, Srnak A, Ebeling A, Kunas G, Catarello J, Burhop K (1992) Synthesis and properties of polymerized, diaspirin cross-linked hemoglobins. Biomater. Artif. Cells Immob. Biotechnol. 20: 253-258.

    Google Scholar 

  • Palaparthy R, Wang H, Gulati A (2000) Current aspects in pharmacology of modified hemoglobins. Adv. Drug Deliv. Rev. 40: 185-198.

    Google Scholar 

  • Snyder SR, Welty EV, Walder RY, Williams LA, Walder JA (1987) HbXL99 alpha: a hemoglobin derivative that is cross-linked between the alpha subunits is useful as a blood substitute. Proc. Natl. Acad. Sci. USA 84: 7280-7284.

    Google Scholar 

  • Spahn DR (2000) Current status of artificial oxygen carriers. Adv. Drug Deliv. Rev. 40: 143-157.

    Google Scholar 

  • Spahn DR, Leone BJ, Reves JG, Pasch T (1994) Cardiovascular and coronary physiology of acute isovolemic hemodilution: a review of nonoxygen-carrying and oxygen-carrying solution. Anesth. Analg. 78: 1000-1021.

    Google Scholar 

  • Winslow RM (2000) Blood substitutes. Adv. Drug Deliv. Rev. 40: 131-142.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Laboratory of Biochemical Engineering, Institute of Process Engineering, P.R. China

    Qiang Yun, Minglei He, Jingxiu Bi, Guanghui Ma & Zhiguo Su

  2. The Xinjiang Technical Institute of Physics & Chemistry, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China

    Wenchao Xing

Authors
  1. Qiang Yun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Minglei He
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wenchao Xing
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jingxiu Bi
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Guanghui Ma
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Zhiguo Su
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Yun, Q., He, M., Xing, W. et al. A novel tetrafunctional reagent for simultaneous intramolecular and intermolecular cross-linking of bovine hemoglobin. Biotechnology Letters 26, 1359–1363 (2004). https://doi.org/10.1023/B:BILE.0000045633.36744.0d

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BILE.0000045633.36744.0d

Search

Navigation