Abstract
Increased expression of recombinant mini-proinsulin in Pichia pastoris in 2.5 l bioreactors was achieved by increasing the cultivation pH from 5.1 to 6.3, by decreasing the temperature from 28 to 22 °C, and by periodical addition of ammonium sulfate and EDTA to the culture broth. Using this procedure, mini-proinsulin reached nearly 0.3 g l−1 in the culture supernatant after 160 h of growth.
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País-Chanfrau, J.M., García, Y., Licor, L. et al. Improving the expression of mini-proinsulin in Pichia pastoris . Biotechnology Letters 26, 1269–1272 (2004). https://doi.org/10.1023/B:BILE.0000044871.80632.f9
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DOI: https://doi.org/10.1023/B:BILE.0000044871.80632.f9