Abstract
A model optical immunosensor was developed to quantify an antibody present in a sample by measuring the fluorescence of Cyanine-5 conjugated with the antibody, using a competitive and a sandwich immunoreaction configuration, with the antigen immobilised in controlled pore glass beads. At pH 2, 94% of the antigen-antibody complex was dissociated, allowing reutilisation. Photobleaching had no effect on the fluorescence. This model system was used to detect Brucella sp. infection and could quantify anti-Brucella sp. antibodies in ovine serum samples in the range from 0.005 to 0.11 mg ml−1.
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Silva, M., Cruz, H., Rossetti, O. et al. Development of an optical immunosensor based on the fluorescence of Cyanine-5 for veterinarian diagnostics. Biotechnology Letters 26, 993–997 (2004). https://doi.org/10.1023/B:BILE.0000030046.96039.e8
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DOI: https://doi.org/10.1023/B:BILE.0000030046.96039.e8