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Quantification of Saccharomyces cerevisiae viability using BacLight

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Abstract

Yeast viability can be accurately quantified using BacLight, a kit which so far has been used only for bacterial analysis. Upon staining, viable cells can be differentiated from non-viable ones by either confocal laser scanning microscopy (CLSM), epifluorescence microscopy, or flow cytometry. Using Saccharomyces cerevisiae as a model, viabilities quantified by CLSM deviated an average of 1.7% from the actual data, and those determined by flow-cytometry by 1.4%.

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References

  • Biswas K, Rieger K, Morschhäuser J (2003) Functional analysis of CaRAP1, encoding the Repressor/activator protein 1 of Candida albicans. Gene 307: 151–158.

    Google Scholar 

  • Boyd AR, Gunasekera TS, Attfield PV, Simic K, Vincent SF, Veal DA (2003) A flow-cytometric method for determination of yeast viability and cell number in a brewery. FEMS Yeast Res. 3: 11–16.

    Google Scholar 

  • Cahill G, Walsh PK, Donnelly D (1999) Improved control of brewery yeast pitching using image analysis. J. Am. Soc. Brew. Chem. 57: 72–78.

    Google Scholar 

  • Dan NP, Visvanathan C, Basu B (2003) Comparative evaluation of yeast and bacterial treatment of high salinity wastewater based on biokinetic coefficients. Bioresour. Technol. 87: 51–56.

    Google Scholar 

  • Haugland RP (1996) Handbook of Fluorescent Probes and Research Chemicals, 9th edn. Eugene, OR: Moleculer Probes Inc.

    Google Scholar 

  • Hope CK, Wilson M (2003) Measuring the thickness of an outer layer of viable bacteria in an oral biofilm by viability mapping. J. Microbiol. Meth. 54: 403–410.

    Google Scholar 

  • Lehtinen J, Virta M, Lilius E (2003) Fluoro-luminometric realtime measurement of bacterial viability and killing. J. Microbiol. Meth. 55: 173–186.

    Google Scholar 

  • Millsap KW, van der Mei HC, Bos R, Busscher HJ (1998) Adhesive interactions between medically important yeasts and bacteria. FEMS Microbiol. Rev. 21: 321–336.

    Google Scholar 

  • Narvhus JA, Gadaga TH (2003) The role of interaction between yeasts and lactic acid bacteria in African fermented milks: a review. Int. J. Food Microbiol. 86: 51–60.

    Google Scholar 

  • Oh KB, Matsuoka H (2002) Rapid viability assessment of yeast cells using vital staining with 2-NBDG, a fluorescent derivative of glucose. Int. J. Food Microbiol. 76: 47–53.

    Google Scholar 

  • Sami M, Ikeda M, Yabuuchi S (1994) Evaluation of the alkaline Methylene Blue staining method for yeast activity determination. J. Ferment. Bioeng. 78: 212–216.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Environmental Biotechnology Laboratory, Department of Civil Engineering The, University of Hong Kong, Pokfulam Road, Hong Kong SAR, China

    Tong Zhang & Herbert H.P. Fang

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  1. Tong Zhang
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  2. Herbert H.P. Fang
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Zhang, T., Fang, H.H. Quantification of Saccharomyces cerevisiae viability using BacLight. Biotechnology Letters 26, 989–992 (2004). https://doi.org/10.1023/B:BILE.0000030045.16713.19

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  • DOI: https://doi.org/10.1023/B:BILE.0000030045.16713.19

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