Abstract
Recombinant Escherichia coli strain C600/pBV-TRAIL (encoding for 114-281 amino acids of TRAIL's soluble fragment) produced a recombinant human tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Using a combined strategy of exponential feeding and pH-stat feeding, high concentrations of biomass (65 g dry wt l−1) and active soluble TRAIL (1.4 g l−1) were obtained within 30 h. The accumulation of acetate, which usually occurs during the process of high-density culture of Escherichia coli and especially in the induction stage of protein synthesis, was avoided.
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Shen, YL., Zhang, Y., Sun, AY. et al. High-level production of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) in high-density cultivation of recombinant Escherichia coli using a combined feeding strategy. Biotechnology Letters 26, 981–984 (2004). https://doi.org/10.1023/B:BILE.0000030043.84226.ac
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DOI: https://doi.org/10.1023/B:BILE.0000030043.84226.ac