Abstract
A mathematical model describing the degradation of arabinoxylans by endo-xylanase during mashing process was developed. Endo-xylanase activities and arabinoxylans concentrations in laboratory scale mashing process at different temperature profiles were measured and then used for identifying the model parameters for Harrington barley malt. The modeling errors range for the final concentration of arabinoxylans in wort was −4% to +11.9%. The model developed was also used for predicting the other three different malts mashing processes in laboratory scale, and the prediction errors ranged from −9.5% to +13.6%. The model prediction accuracy for industrial scale mashing process was lower than that in laboratory scale. The simulation results showed that, a lower concentration of arabinoxylans could be achieved when maintaining the mashing-in at 45 °C and prolonging the mashing-in time.
Similar content being viewed by others
References
Angelino SAGF (1991) Non-starch polysaccharides in barley and malt: a mass balance of flour fractionation. J. Cereal Sci. 14: 73–83.
Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J. Biotechnol. 23: 257–270.
Barrett J, Bathgate GN, Clapperton JF (1975) The composition of fine particles which affect mash filtration. J. Inst. Brew. 81: 31–36.
Coote N, Kirsop BH (1976) A haze consisting largely of pentosan. J. Inst. Brew. 82: 34.
Debyser W, Derdelinckx G, Delcour JA (1997) Arabinoxylan and arabinoxylan hydrolyzing activities in barley malt and worts derived from them. J. Cereal Sci. 26: 67–74.
Egylst HN, Cummings JH (1984) Simplified method for the determination of total non-starch polysaccharides by gas-liquid chromatography of constituent sugars as alditol acetates. Analyst 109: 937–942.
Henry RJ (1986) Genetic and environmental variation in the pentosan and β-glucan contents of barley and their relation to malting quality. J. Cereal Sci. 4: 269–277.
Koljonen T, Hämäläinen JJ, Sjöholm K, Pietilä K (1995) A model for the prediction of fermentable sugar concentrations during mashings. J. Food Eng. 26: 329–350.
Koljonen T, Kettunen A, Sjöholm K, Pietilä KA, Hämäläinen JJ (1992) Simulation of enzyme kinetics during malt mshing-a new parameter estimation method. In Proceedings of the 1st IEEE Conference on Contr. Appl. Piscataway, NJ: The Institute of Electrical and Electronics Engineers, Vol. 1, pp. 90–5. ISBN 0 7803 0047 5.
Marc A, Engasser JM, Moll M, Flayeux R (1983) A kinetic model of starch hydrolysis by α-and β-amylase during mashing. Biotechnol. Bioeng. 28: 481–496.
Rice RC (1960) Split Runge-Kutta methods for simultaneous equations. J. Res. Nat. Bur. Stand. 64B: 151–170.
Schwarz PB, Han JY (1995) Arabinoxylan content of commercial beers. J. Am. Soc. Brew. Chem. 53: 157–159.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Li, Y., Lu, J., Gu, G. et al. Mathematical modeling for prediction of endo-xylanase activity and arabinoxylans concentration during mashing of barley malts for brewing. Biotechnology Letters 26, 779–785 (2004). https://doi.org/10.1023/B:BILE.0000025877.37767.00
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000025877.37767.00