Abstract
The maximum volumetric productivity of β-galactosidase by a Kluyveromyces marxianus mutant, grown on lactose/corn steep liquor medium for 3 d, was 150 IU l−1 h−1 which is twice that of the parent organism. During product formation, mutated cells provided more resistance against thermal inactivation.
Similar content being viewed by others
References
Al-Asheh S, Duvniak Z (1994) Characteristics of phytase produced by Aspergillus carbonarius NRC 401121 in canola meal. Acta Biotechnol. 14: 223–237.
Beccerra M., Prado SD, Rodriguez-Balmonte E, Cerdan ME, Siso MIG (2002) Metabolic engineering for direct lactose utilization by Saccharomyces cerevisiae. Biotechnol. Lett. 24: 1391–1396.
Belem MAF, Lee BH (1998) Production of bioingredients from Kluyveromycess marxianus grown on whey: an alternative. Crit. Rev. Food Sci. Nutr. 38: 565–598.
Converti A, Del Borghi M (1997) Simultaneous effects of immobilization and substrate protection on the thermodynamics of glucose isomerase activity and inactivation. Enzyme Microb. Technol. 21: 511–517.
Converti A, Dominguez JM (2001) Influence of temperature and pH on xylitol production from xylose by Debarryomyces hansenii. Biotechnol. Bioeng. 75: 39–45.
Declerck N, Machius M, Joyet P, Wiegand G, Huber R, Gaillardin C (2003) Hyperthermostabilization of Bacillus licheniformis alphaamylase and modulation of its stability over a 50 °C temperature range. Protein Eng. 16: 287–293.
Domingues L, Teixiera JA, Pentitila M, Lima N (2002). Construction of a flocculent Saccharomyces cerevisiae strain secreting high levels of Aspergillus niger ?-galactosidase. Appl. Microbiol. Biotechnol. 58: 645–650.
Furlan AS, Schneider ASL, Merckle R, Carvalho-Johans MF, Jonas R (2000) Formulation of lactose-free, low-cost culture medium for the production of ?-galactosidase by Kluyveromyces marxianus. Biotechnol. Lett. 22: 1195–1198.
Gelinger UM, Guckel R, Hoffmann M, Wolf DH, Hilt W (1997) Yeast cycloheximide-resistant crl mutants are proteasome mutants defective in protein degradation. Mol. Biol. Cell. 8: 2487–2499.
Lawford HG, Rousseau JD (1993) Mannose fermentation by ethanologenic recombinants of Escherichia coli. Biotechnol. Lett. 15: 615–620.
Rajoka MI, Bashir A, Malik KA (1997) Mutagenesis of Cellulomonas biazotea for enhanced production of xylanases. Bioresour. Technol. 62: 99–108.
Rajoka MI, Khan S, Shahid R (2003) Kinetics and regulation studies of the production of ?-galactosidase from Kluyveromyces marxianus grown on different substrates. Food Technol. Biotechnol. 41: 315–320.
Rashid MH, Siddiqui KS (1998) Thermodynamic and kinetic study of stability of the native and chemically modified ?-glucosidase from Aspergillus niger. Proc. Biochem. 33: 109–115.
Siddiqui KS, Rashid MH, Rajoka MI (1997) Kinetic analysis of the active site of an intracellular ?-glucosidase from Cellulomonas biazotea. Folia Microbiol. 42: 53–58.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Rajoka, M., Latif, F., Khan, S. et al. Kinetics of improved productivity of β-galactosidase by a cycloheximide-resistant mutant of Kluyveromyces marxianus . Biotechnology Letters 26, 741–746 (2004). https://doi.org/10.1023/B:BILE.0000024089.52462.98
Issue Date:
DOI: https://doi.org/10.1023/B:BILE.0000024089.52462.98