Abstract
The fluorescence emission wavelength of α-chymotrypsin (CT) correlated with its enantioselectivity (E value) for the resolution of dl-tyrosine ethyl ester. The changes in the E value of the CT due to the changes in the solvent composition were closely related to its fluorescence properties (Δλem), which were most probably associated with the structural modification of the enzyme. A linear relationship was established between E value and Δλem in aqueous acetonitrile with high correlation coefficients (r=0.94).
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Kijima, T., Izumi, T. Fluorescence spectroscopic study of α-chymotrypsin relevant to the enantioselectivity for optical resolution of amino acid esters in organic solvents. Biotechnology Letters 26, 655–658 (2004). https://doi.org/10.1023/B:BILE.0000023025.35516.12
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DOI: https://doi.org/10.1023/B:BILE.0000023025.35516.12