Skip to main content
SpringerLink
Log in

Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

Giant catfish growth hormone (gcGH) cDNA was cloned and expressed in E. coli. The expected 20.5 kDa protein corresponded to the mature gcGH and was efficiently expressed. This protein was produced as inclusion bodies and comprised about 20% of total cellular proteins. The recombinant hormone promoted growth when injected intramuscularly or intraperitoneally into goldfish (Carassius auratus) at 0.1 or 1 μg soluble gcGH per g fish body wt per week. In addition, the recombinant gcGH inclusions had growth-promoting activity similar to that of the soluble form when the fish was received either by intraperitoneal injection or by oral administration.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Chen HT, Pan FM, Chang WC (1988) Purification of duck growth hormone and cloning of the complementary DNA. Biochim. Biophys. Acta 949: 247-251.

    Google Scholar 

  • Kawauchi H, Moriyama S, Yasuda A, Yamaguchi K, Shirahata K, Kubota J, Hirano T (1986) Isolation and characterization of chum salmon growth hormone. Arch. Biochem. Biophysic. 244: 542-552.

    Google Scholar 

  • Kimura A (1991) Microbial production of tuna growth hormone. Crit. Rev. Biotechnol. 11: 113-127.

    Google Scholar 

  • Langley KE, Lai PH, Wypych J, Everett RR, Berg TF, Krabill LF, Davis JM, Souza LM (1987) Recombinant DNA derived bovine growth hormone from E. coli: 2. biochemical, biophysical, immunological and biological comparison with the pituitary hormone. Eur. J. Biochem. 163: 323-330.

    Google Scholar 

  • Lemaire C, Warit S, Panyim S (1994) Giant catfish Pangasianodon gigas growth hormone-encoding cDNA: cloning and sequencing by one-sided polymerase chain reaction. Gene 149: 271-276.

    Google Scholar 

  • Miller WL, Martial JA, Baxter JD (1980) Molecular cloning of DNA complementary to bovine growth hormone mRNA. J. Biol. Chem. 255: 7521-7524.

    Google Scholar 

  • Moriyama S, Yamamoto H, Sugimoto S, Abe T, Hirano T, Kawauchi H (1993) Oral administration of recombinant salmon growth hormone to rainbow trout, Oncorhynchus mykiss. Aquaculture 112: 99-106.

    Google Scholar 

  • Pookaswan T (1969) Pangasianodon gigas Chevey. J. Fish. (Department of Fisheries, Thailand) 21: 431-452.

    Google Scholar 

  • Rentier-Dulue F, Swenner D, Philippart JC, L-Hoir C, Lion M, Benrubi O, Martial JA (1989) Tilapia growth hormone: molecular cloning of cDNA and expression in E. coli.DNA 8: 271-278.

    Google Scholar 

  • Sato N, Murata K, Watanabe K, Hayami T, Kariya Y, Sakaguchi M, Kimura S, Nonaka M, Kimura A (1988) Growth-promoting activity of tuna growth hormone and expression of tuna growth hormone cDNA in Escherichia coli. Biotechnol. Appl. Biochem. 10: 385-393.

    Google Scholar 

  • Tsai H, Lin K, Kuo J, Chen S (1995) Highly efficient expression of fish growth hormone by Escherichai coli cells. Appl. Environ. Microbiol. 61: 4116-4119.

    Google Scholar 

  • Yasuda A, Yamaguchi K, Noso T, Papkoff H, Palenov AL, Nicoll CS, Kawauchi H (1992) The complete amino acid sequence of growth hormone from sturgeon (Acipencer guldenstadti). Biochim. Biophys. Acta 1120: 297-304.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency, 113 Phaholyothin Road, Klong 1, Klong Luang, Pathumthani, 12120, Thailand

    Boonhiang Promdonkoy & Saradee Warit

  2. Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhonpathom, 73170, Thailand

    Sakol Panyim

Authors
  1. Boonhiang Promdonkoy
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Saradee Warit
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Sakol Panyim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Promdonkoy, B., Warit, S. & Panyim, S. Production of a biologically active growth hormone from giant catfish (Pangasianodon gigas) in Escherichia coli . Biotechnology Letters 26, 649–653 (2004). https://doi.org/10.1023/B:BILE.0000023024.27549.6d

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BILE.0000023024.27549.6d

Search

Navigation