Abstract
An amperometric biosensor was developed to estimate galactose in human blood serum. Monolayers of poly(3-hexyl thiophene) were placed on glass plates coated with indium tin oxide formed by dispensing a mixed solution of stearic acid in chloroform on to a water sub-phase. Galactose oxidase was mixed with poly(3-hexyl thiophene)/stearic acid in chloroform and dispensed on to the air-water interface of Langmuir–Blodgett trough. These monolayers were transferred on to glass plates which were used as working electrodes with platinum as a reference electrode. The amperometric galactose biosensor thus fabricated had a linear response from 0.05 to 0.5 g galactose l−1 in blood serum. The normal level in blood is < 0.05 g galactose l−1 in adults and 0–0.2 g galactose l−1 in infants. In case of galactosemia, this increases to above 0.2 g galactose l−1 in infants.
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Sharma, S.K., Singhal, R., Malhotra, B. et al. Biosensor based on Langmuir–Blodgett films of poly(3-hexyl thiophene) for detection of galactose in human blood. Biotechnology Letters 26, 645–647 (2004). https://doi.org/10.1023/B:BILE.0000023023.19651.01
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DOI: https://doi.org/10.1023/B:BILE.0000023023.19651.01