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Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions

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Abstract

A monoclonal antibody produced by hydridoma cell line, ATCC HB8209, was used to detect and purify erythropoietin synthesized in a cell-free system. The antibody was raised against the N-terminal 20 residues of erythropoietin. It retained anti-erythropoietin activity in 6 m urea in which most of the cell-free synthesized erythropoietin became soluble and gave an enhanced activity of the antibody.

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Authors and Affiliations

  1. School of Chemical Engineering, College of Engineering, Seoul National University, Seoul, 151-742, Korea

    Taek Jin Kang, Tae Wan Kim & Cha Yong Choi

  2. Interdisciplinary Program for Biochemical Engineering and Biotechnology, College of Engineering, Seoul National University, Seoul, 151-742, Korea

    Hui Kyoung Song, Jin Ho Ahn & Cha Yong Choi

  3. School of Chemical Engineering and Bioengineering, College of Engineering, Ulsan University, Ulsan, 680-749, Korea

    Yeo Joon Yoon

Authors
  1. Taek Jin Kang
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  2. Hui Kyoung Song
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  3. Jin Ho Ahn
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  4. Tae Wan Kim
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  5. Yeo Joon Yoon
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  6. Cha Yong Choi
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Jin Kang, T., Kyoung Song, H., Ho Ahn, J. et al. Enhanced immunodetection of cell-free synthesized erythropoietin under denaturing conditions. Biotechnology Letters 26, 641–644 (2004). https://doi.org/10.1023/B:BILE.0000023022.60035.1e

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  • DOI: https://doi.org/10.1023/B:BILE.0000023022.60035.1e

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