Skip to main content
SpringerLink
Log in

Cloning and characterization of a sigma 70 homologue gene, sigA from Corynebacterium ammoniagenes

  • Published:
Biotechnology Letters Aims and scope Submit manuscript

Abstract

A sigma 70-like gene, sigA, has been identified from Corynebacterium ammoniagenes. The sigA gene encodes a polypeptide of 467 amino acids with a calculated molecular mass of 52 036 Da. The deduced amino acid sequence preserves the common motifs of the primary sigma factors and shows very high similarity to those of SigA (σA) homologues from high G+C Gram-positive bacteria, which suggest that the sigA gene encodes the primary sigma factor. The sigA gene is transcribed as a monocistronic mRNA of 2 kb and its mRNA occurs during the exponential growth phase and decays rapidly on entry into the stationary phase. The open reading frame encoding polyphosphate glucokinase-like protein is closely linked to the sigA gene.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bashyam MD, Kaushal D, Dasgupta SK, Tyagi AK (1996) A study of mycobacterial transcriptional apparatus: identification of novel features in promoter elements. J. Bacteriol. 178: 4847–4853.

    Google Scholar 

  • Borodovsky M, McIninch J (1993) GeneMark: parallel gene recognition for both DNA strands. Comp. Chem. 17: 123–133.

    Google Scholar 

  • Campbell EA, Muzzin O, Chlenov M, Sun JL, Olson CA, Weinman O, Trester-Zedlitz ML, Darst SA (2002) Structure of the bacterial RNA polymerase promoter specificity sigma subunit. Mol. Cell. 9: 527–539.

    Google Scholar 

  • Fujio T, Nishi T, Ito S, Maruyama A (1997) High level expression of XMP aminase in Escherichia coli and its application for the industrial production of 5?-guanylic acid. Biosci. Biotechnol. Biochem. 61: 840–845.

    Google Scholar 

  • Halgasova N, Bukovska G, Timko J, Kormanec J (2001) Cloning and transcriptional characterization of two sigma factor genes, sigA and sigB, from Brevibacterium flavum. Curr. Microbiol. 43: 249–254.

    Google Scholar 

  • Halgasova N, Bukovska G, Ugorcakova J, Timko J, Kormanec J (2002) The Brevibacterium flavum sigma factor SigB has a role in the environmental stress response. FEMS Microbiol. Lett. 216: 77–84.

    Google Scholar 

  • Hsieh PC, Shenoy BC, Samols D, Phillips NF (1996) Cloning, expression, and characterization of polyphosphate glucokinase from Mycobacterium tuberculosis. J. Biol. Chem. 271: 4909–4915.

    Google Scholar 

  • Kamada N, Yasuhara A, Takano Y, Nakano T, Ikeda M (2001) Effect of transketolase modifications on carbon flow to the purinenucleotide pathway in Corynebacterium ammoniagenes. Appl. Microbiol. Biotechnol. 56: 710–717.

    Google Scholar 

  • Koizumi S, Yonetani Y, Maruyama A, Teshiba S (2000) Production of riboflavin by metabolically engineered Corynebacterium ammoniagenes. Appl. Microbiol. Biotechnol. 53: 674–679.

    Google Scholar 

  • Lonetto M, Gribskov M, Gross CA (1992) The sigma 70 family: sequence conservation and evolutionary relationships. J. Bacteriol. 174: 3843–3849.

    Google Scholar 

  • Mangan JA, Sole KM, Mitchison DA, Butcher PD (1997) An effective method of RNA extraction from bacteria refractory to disruption, including mycobacteria. Nucl. Acids Res. 25: 675–676.

    Google Scholar 

  • Mori H, Iida A, Fujio T, Teshiba S (1997) A novel process of inosine 5?-monophosphate production using overexpressed guanosine/inosine kinase. Appl. Microbiol. Biotechnol. 48: 693–698.

    Google Scholar 

  • Murakami KS, Masuda S, Campbell EA, Muzzin O, Darst SA (2002) Structural basis of transcription initiation: an RNA polymerase holoenzyme-DNA complex. Science 296: 1285–1290.

    Google Scholar 

  • Oguiza JA, Marcos AT, Malumbres M, Martin JF (1996) Multiple sigma factor genes in Brevibacterium lactofermentum: characterization of sigA and sigB. J. Bacteriol. 178: 550–553.

    Google Scholar 

  • Oguiza JA, Marcos AT, Martin JF (1997) Transcriptional analysis of the sigA and sigB genes of Brevibacterium lactofermentum. FEMS Microbiol. Lett. 153: 111–117.

    Google Scholar 

  • Patek M, Nesvera J, Guyonvarch A, Reyes O, Leblon G (2003) Promoters of Corynebacterium glutamicum. J. Biotechnol. 104: 311–323.

    Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory Press.

    Google Scholar 

  • Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucl. Acids Res. 22: 4673–4680.

    Google Scholar 

  • Wosten MM (1998) Eubacterial sigma-factors. FEMS Microbiol. Rev. 22: 127–150.

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Biomedical Science and Engineering, and Genome Research Center, Inje University, Gimhae, 621-749, Korea

    Baek Rak Lee

  2. Indang Institute of Molecular Biology, Inje University, Seoul, 100-032, Korea

    Jeong Hyun Kim

Authors
  1. Baek Rak Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Jeong Hyun Kim
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, B.R., Kim, J.H. Cloning and characterization of a sigma 70 homologue gene, sigA from Corynebacterium ammoniagenes . Biotechnology Letters 26, 589–594 (2004). https://doi.org/10.1023/B:BILE.0000021962.16204.08

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:BILE.0000021962.16204.08

Search

Navigation