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Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid

Biotechnology Letters volume 26, pages 521–523 (2004)Cite this article

Abstract

Nodal explants with lateral buds and leaf-derived suspension cultures of sweet basil, Ocimum basilicum L., were cultured in 5 l airlift bioreactors for three weeks, thereby increasing the fresh wt of suspension cultures 2.5-fold. Rosmarinic acid accumulated at 29 μg g−1 dry wt in the suspension culture but, for micropropagated plants, it reached 178 μg g−1 dry wt.

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Affiliations

  1. Laboratory of Plant Physiology, Faculty of Agricultural Biotechnology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Spiridon Kintzios, Haralambos Kollias, Evangelos Straitouris & Olga Makri

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  1. Spiridon Kintzios
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  2. Haralambos Kollias
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  3. Evangelos Straitouris
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  4. Olga Makri
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Kintzios, S., Kollias, H., Straitouris, E. et al. Scale-up micropropagation of sweet basil (Ocimum basilicum L.) in an airlift bioreactor and accumulation of rosmarinic acid. Biotechnology Letters 26, 521–523 (2004). https://doi.org/10.1023/B:BILE.0000019561.89044.30

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  • DOI: https://doi.org/10.1023/B:BILE.0000019561.89044.30

  • airlift bioreactor
  • micropropagation
  • Ocimum basilicum
  • rosmarinic acid