Abstract
Two novel non-reducing oligosaccharides together with tri- and tetra-saccharides were synthesized by transfructosylation activity from sucrose as a donor and cellobiose or cellotriose as an acceptor with a purified β-fructofuranosidase from Arthrobacter globiformis IFO 3062, and these oligosaccharides were identified as O-β-d-glucopyranosyl-(1\to4)-α-d-glucopyranosyl-(1\to2)-α,β-d-fructofuranoside and O-β-d-glucopyranosyl-(1\to4)-β-d-glucopyranosyl-(1\to4)-α-d-glucopyranosyl-(1\to2)-α, β-d-fructofuranoside by spectrometric analyses. Both oligosaccharides were stable under condition at 100 °C for 30 min, and showed no degradation at pH 2.
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Win, T.T., Isono, N., Kusnadi, Y. et al. Enzymatic synthesis of two novel non-reducing oligosaccharides using transfructosylation activity with β-fructofuranosidase from Arthrobacter globiformis . Biotechnology Letters 26, 499–503 (2004). https://doi.org/10.1023/B:BILE.0000019557.44196.63
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DOI: https://doi.org/10.1023/B:BILE.0000019557.44196.63