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Increasing glutathione formation by functional expression of the γ-glutamylcysteine synthetase gene in Saccharomyces cerevisiae

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Abstract

A recombinant plasmid, pGMF, containing a γ-glutamylcysteine synthetase gene (GSH-I) from Saccharomyces cerevisiae, was constructed with a copper-resistance gene as the selection marker and was introduced into S. cerevisiae YSF-31. The glutathione content of the recombinant strain was 1.5-fold (13.1 mg g dry cells−1) of that in the host strain.

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Authors and Affiliations

  1. The Center of Biotechnology, Institute of Microbiology, Academia Sinica, Beijing, 100080, P.R. China

    Xiuying Fan, Xiuping He, Xuena Guo, Na Qu & Borun Zhang

  2. Department of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300222, P.R. China

    Xiuying Fan & Changlu Wang

Authors
  1. Xiuying Fan
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  2. Xiuping He
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  3. Xuena Guo
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  4. Na Qu
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  5. Changlu Wang
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  6. Borun Zhang
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Fan, X., He, X., Guo, X. et al. Increasing glutathione formation by functional expression of the γ-glutamylcysteine synthetase gene in Saccharomyces cerevisiae . Biotechnology Letters 26, 415–417 (2004). https://doi.org/10.1023/B:BILE.0000018261.14427.e8

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  • DOI: https://doi.org/10.1023/B:BILE.0000018261.14427.e8

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