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Bacillus subtilis transcriptional regulators interaction

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Abstract

Bacillus subtilis aprE gene codes for the extracellular protease subtilisin. Its expression is controlled by AbrB, DegU, Hpr, SinI, SinR and Spo0A transition state protein regulators. To determine in vivo the protein–protein interactions among these regulators, we used the LexA-based bacterial genetic two-hybrid system. Our results show homo-dimerization to all the analyzed proteins and hetero-dimerization between SinR-SinI and SinR-Hpr.

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Authors and Affiliations

  1. Departamento de Biotecnología Marina, Centro de Investigación Científica y de Educación Superior de Ensenada (CICESE), Km 107, Ensenada BC, México

    Alejandro Sánchez

  2. Biotecnología Marina, P.O. Box 434844, San Diego, CA, 92143-4844, USA

    Jorge Olmos

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  1. Alejandro Sánchez
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  2. Jorge Olmos
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Sánchez, A., Olmos, J. Bacillus subtilis transcriptional regulators interaction. Biotechnology Letters 26, 403–407 (2004). https://doi.org/10.1023/B:BILE.0000018259.66762.ed

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