Abstract
A fibrinolytic enzyme, myulchikinase, from a Korean seasoning ingredient, myul-chi-jeot-gal, has been purified to electrophoretic homogeneity. The molecular mass of the myulchikinase was estimated to about 28 kDa by SDS-PAGE and gel filtration. Amino acid sequence of the NH2-terminal of myulchikinase showed significant homology with other fibrinolytic enzymes including trypsin from starfish, katsuwokinase, and rat pancreatic elastase II. The purified myulchikinase hydrolyzed various synthetic substrates with different substrate specificity and cytotoxic to the tumor cell lines.
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Altschul SF, Madden TL, Scaffer AA, Zhang J, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl. Acids Res. 25: 3389–3402.
Astrup T, Müllertz S (1952) The fibrin plate method for estimating fibrinolytic activity. Arch. Biochem. Biophys. 40: 346–351.
Espevik T, Nissen-Meyer J (1986) A highly sensitive cell line, WEHI164 clone 13, for measuring cytotoxic factor/tumor necrosis factor from human monocytes. J. Immunol. Methods 95: 99–105.
Estell DA, Laskowski Jr. M (1980) Dermasterias imbricata trypsin 1: an enzyme which rapidly hydrolyzes the reactive-site peptide bonds of protein trypsin inhibitors. Biochemistry 19: 124–131.
Jacobs M, Eliasson M, Uhlen M, Flock JI (1985) Cloning, sequencing and expression of subtilisin Carlsberg from Bacillus licheniformis. Nucl. Acids Res. 13: 8913–8926.
Kim WK, Choi K, Kim Y, Park H, Choi J, Lee Y, Oh H, Kwon I, Lee S (1996) Purification and characterization of a fibrinolytic enzyme produced from Bacillus strain CK 11-4 screened from Chungkook-Jang. Appl. Environ. Microbiol. 62: 2482–2488.
Liotta LA, Steeg PS, Stetler-Stevenson WG (1991) Cancer metastasis and angiogenesis: an imbalance of positive and negative regulation. Cell 64: 327–336.
Park YD, Kim LW, Min BG, Seo JW, Jeong JM (1998) Rapid purification and biochemical characteristics of lumbrokinase III from earthworm for use as a fibrinolytic agent. Biotechnol. Lett. 20: 169–172.
Sumi H, Hamada H, Nakanishi K, Hiratani H (1990) Enhancement of the fibrinolytic activity in plasma by oral administration of NK. Acta Haematol. 84: 139–143.
Sumi H, Hamada H, Tsushima H, Mihara H, Muraki H (1987) A novel fibrinolytic enzyme (Nattokinase) in the vegetable cheese natto: a typical and popular soybean food in the Japanese diet. Experimentia 43: 1110–1111.
Sumi H, Nakajima N, Yatagai C (1995) A unique strong fibrinolytic enzyme (katsuwokinase) in skipjack 'Shiokara', a Japanese traditional fermented food. Comp. Biochem. Physiol. 112: 543–547.
Szilagyi CM, Sarfati P, Pradayrol L, Morisset J (1995) Purification, characterization and substrate specificity of rat pancreatic elastase II. Biochim. Biophys. Acta. 1251: 55–65.
Toki N, Sumi H, Sasaki K, Boreisha I, Robbins KC (1985) Transport of urokinase across the intestinal tract of normal human subjects with stimulation of synthesis and/or release of urokinase-type proteins. J. Clin. Invest. 75: 1212–1222.
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Jeong, YK., Yang, W.S., Kim, K.H. et al. Purification of a fibrinolytic enzyme (myulchikinase) from pickled anchovy and its cytotoxicity to the tumor cell lines. Biotechnology Letters 26, 393–397 (2004). https://doi.org/10.1023/B:BILE.0000018257.18617.6d
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DOI: https://doi.org/10.1023/B:BILE.0000018257.18617.6d