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Nisin Production by Lactococcus Lactis Subsp. lactis under Nutritional Limitation in Fed-Batch Culture

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Abstract

Nisin production was improved under nutritional limitation in fed-batch culture of Lactococcus lactis. Nisin titre reached 3887 IU ml−1 by a slow feeding of sucrose and 4131 IU ml−1 by slow feeding of nitrogen source, which were, respectively, 64% and 74% above the values in batch culture.

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References

  • De Vuyst L, Vandamme EJ (1992a) Influence of the carbon source on nisin production in Lactococcus lactis subsp. Lactis batch fermentations. J. Gen. Microbiol. 138: 571–578.

    Google Scholar 

  • De Vuyst L, Vandamme EJ (1992b) Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. Lactis batch fermentations using a complex medium. Appl. Microbiol. Biotechnol. 40: 17–22.

    Google Scholar 

  • De Vuyst L, Callewaert Raf, Crabbe Kurt (1996) Primary metabolite kinetics of bacteriocin biosynthesis by Lactobacillus amylovorus and evidence for stimulation of bacteriocin production under unfavourable growth conditions. Microbiology 142: 817–827.

    Google Scholar 

  • Egorov NS, Baranova IP, Kozlova Yui (1971) Optimization of the nutrient medium composition for the production of the antibiotic nisin by streptococcus lactis. Mikrobiologiya 40: 993–998.

    Google Scholar 

  • Gross E, Morell JL (1971) The structure of nisin. J. Am. Chem. Soc. 93: 4634–4639.

    Google Scholar 

  • Hurst A (1981) Nisin. Adv. Appl. Microbiol. 27: 85–123.

    Google Scholar 

  • James LS, Larry LM (1986) Partial characterization of the genetic basis for sucrose metabolism and nisin production in streptococcus lactis. Appl. Environ. Microbiol. 51: 57–64.

    Google Scholar 

  • Matsusaki H, Endo N, Sonomoto K, Ishizaki A (1996) Lantibiotic nisin Z fermentative production by Lactococcus lactis IO-1-relationship between production of the lantibiotic and lactate and cell-growth. Appl. Microbiol. 45: 36–40.

    Google Scholar 

  • Steinsholt K, Calbert HE (1960) A rapid colorimetric method for the determination of lactic acid in milk and milk products. Milchwissenschaft 15: 7–11.

    Google Scholar 

  • Tramer J, Fowler GG (1964) Estimation of nisin in foods. J. Sci. Food. Agric. 15: 522–528.

    Google Scholar 

  • Van't Hul JS, Gibbons WR (1997) Neutralization/recovery of lactic acid from Lactococcus lactis: effects on biomass, lactic acid, and nisin production. World J. Microbiol. Biotechnol. 13: 527–532.

    Google Scholar 

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Authors and Affiliations

  1. State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, P.O. Box 353, Beijing, 100080, P.R. China

    Wenhua Lv, Wei Cong & Zhaoling Cai

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  1. Wenhua Lv
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  2. Wei Cong
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  3. Zhaoling Cai
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Lv, W., Cong, W. & Cai, Z. Nisin Production by Lactococcus Lactis Subsp. lactis under Nutritional Limitation in Fed-Batch Culture. Biotechnology Letters 26, 235–238 (2004). https://doi.org/10.1023/B:BILE.0000013721.78288.1d

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  • DOI: https://doi.org/10.1023/B:BILE.0000013721.78288.1d

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