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Cloning and Sequence Analysis of the dhaT Gene of the 1,3-Propanediol Regulon from Klebsiella Pneumoniae

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Abstract

1,3-Propanediol oxidoreductase encoded by dhaT gene, a gene of 1,3-propanediol regulon, is important in converting glycerol to 1,3-propanediol in Klebsiella pneumoniae. DhaT gene was amplified from the genome of K. pneumoniae, sequenced and its amino acid sequence deduced. A predicted secondary structure and 3D-structural model was constructed by homology modelling. Based on these results, we infer that 1,3-propanediol oxidoreductase belongs to NAD(P)-dependent alcohol dehydrogenase group III of iron-activated dehydrogenases.

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Yuanyuan, Z., Yang, C. & Baishan, F. Cloning and Sequence Analysis of the dhaT Gene of the 1,3-Propanediol Regulon from Klebsiella Pneumoniae . Biotechnology Letters 26, 251–255 (2004). https://doi.org/10.1023/B:BILE.0000013715.04456.0a

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  • DOI: https://doi.org/10.1023/B:BILE.0000013715.04456.0a

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