Abstract
It has been previously shown that exposing Drosophila melanogaster flies to hypergravity (3g or 5g) at a young age for 2 weeks increases male longevity, resistance to heat in both sexes, and delays behavioural ageing, but the causes of these effects are unknown. We hypothesised that these flies could be well protected against free radical attacks and, if this protection persists after removal from hypergravity, can better resist free radicals and finally live longer than flies that have always lived at 1g. If so, the activity of enzymes detoxifying free radicals superoxide dismutase and catalase should be increased in flies that have lived in hypergravity. Results showed that no effect of hypergravity on the activity of these enzymes was observed at 2, 4 or 6 weeks of age. The greater longevity of male flies that have lived in hypergravity at a young age thus cannot be explained by the activity changes of these major antioxidant enzymes.
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References
Byun DS, Venkatraman JT, Yu BP and Fernandes G (1995) Modulation of antioxidant activities and immune response by food restriction in aging Fischer-344 rats. Aging Clin Exp Res 7: 40–48
Daligcon BC and Oyama J (1975) Increased uptake and utilization of glucose by diaphragms of rats exposed to chronic centrifugation. Am J Physiol 228: 742–746
Durusoy M, Diril N and Bozcuk N (1995) Age-related activity of catalase in different genotypes of Drosophila melanogaster.Exp Geront 30: 77–86.
Harman D (1956) Aging: a theory based on free radical and radiation chemistry. J Geront 11: 298–300
Johnson TE, Lithgow GJ and Murakami S (1996) Hypothesis: interventions that increase the response to stress offer the potential for effective life prolongation and increased health. J Geront. Biol Sci 51A: B392–B395
Hercus MJ, Loeschcke V and Rattan SIS (2003) Lifespan extension of Drosophila melanogaster through hormesis by repeated mild heat stress. Biogerontology 4: 149–156
Khazaeli AA, Tatar M, Pletcher SD and Curtsinger JW (1997) Heat-induced longevity extension in Drosonhila. I Heat treatment, mortality, and thermotolerance. J Gerontol Biol Sci 52A: B48–B52
Koizumi A, Weindruch R and Walford RL (1987) Influences of dietary restriction and age on liver enzyme activities and lipid peroxidation in mice. J Nutr 117: 361–367
Le Bourg E (1987) The rate of living theory. Spontaneous locomotor activity, aging and longevity in Drosophila melanogaster. Exp Geront 22: 359–369
Le Bourg E (2001) Oxidative stress, aging and longevity in Drosophila melanogaster. FEBS Letters 498: 183–186
Le Bourg E (2002) Are stress resistance and longevity really linked in normal living conditions? Gerontology 48: 109–111
Le Bourg E and Minois N (1996) Failure to confirm increased longevity in Drosophila melanogaster flies submitted to a food restriction procedure. J Geront Biol Sci 51A: B280–B283
Le Bourg E and Minois N (1997) Increased longevity and resistance to heat shock in Drosophila melanogaster flies exposed to hypergravity. C R Acad Sci Paris 320: 215–221
Le Bourg E and Minois N (1999) A mild stress, hypergravity exposure, postpones behavioral aging in Drosophila melanogaster. Exp Geront 34: 157–172
Le Bourg E, Minois N, Bullens P and Baret P (2000) A mild stress due to hypergravity exposure at young age increases longevity in Drosophila melanogaster males. Biogerontology 1: 145–155
Le Bourg E, Valenti P, Lucchetta P and Payre F (2001) Effects of mild heat shocks at young age on aging and longevity in Drosophila melanogaster. 1. Fecundity}. Gerontology 35: 235–243
Massie HR, Aiello VR and Williams TR (1980) Changes in superoxide dismutase activity and copper during development and ageing in the fruit fly Drosophila melanogaster. Mech Aging Dev 12: 179–286
Massie HR and Baird MB (1976) Catalase levels in Drosophila and the lack of induction by hypolipidemic compounds. A brief note. Mech Ageing Dev 5: 39–43
Minois N and Le Bourg E (1999) Resistance to stress as a function of age in Drosophila melanogaster living in hypergravity. Mech Ageing Dev 109: 53–64
Minois N, Guinaudy MJ, Payre F and Le Bourg E (1999)HSP70 induction may explain the long-lasting resistance to heat of Drosophila melanogaster having lived in hypergravity. Mech Ageing Dev 109: 65–77
Minois N and Rattan SIS (2003) Hormesis in aging and longevity. In: Rattan SIS (ed) Modulating aging and longevity, pp. 127–137. Kluwer Academic Publishers, Dordrecht, The Netherlands
Mockett RJ, Bayne ACV Kwong LK, Orr WC and Sohal RS (2003) Ectopic expression of catalase in Drosophila mitochondria increases stress resistance but not longevity. Free Rad Biol Med 34: 207–217
Nicolosi RJ, Baird MB, Massie HR and Samis HV (1973) Senescence in Drosophila-II Renewal of catalase activity in flies of different ages. Exp Geront 8: 101–108
Niedzwiecki A, Reveillaud I and Fleming JE (1992) Changes in superoxide dimutase and catalase in aging heat-shocked Drosophila. Free Rad Res Comms 17: 355–367
Orr WC and Sohal RS (1992) The effects of catalase gene overexpression on life span and resistance to oxidative stress in transgenic Drosophila melanogaster. Arch Biochem Biophys 297: 35–41
Orr WC and Sohal RS (2003) Does overexpression of Cu,Zn-SOD extend life span in Drosophila melanogaster? Exp Geront 38: 227–230
Oyama J and Chan L (1973) Oxygen consumption and carbon dioxide production in rats during acute centrifugation stress and after adaptation to chronic centrifugation. Fed Proc 32: 392
Pearl R (1928) The Rate of Living. Knopf London. Simon AF, Shih C, Mack A and Benzer S (2003) Steroid control of longevity in Drosophila melanogaster. Science 299: 1407–1410
Sohal RS, Allen L and Orr WC (1990) Effect of age on superoxide dismutase, catalase, glutathione reductase, inorganic peroxides, TBA-reactive material, GSH/GSSG, NADPH/ NADP+ and NADH/NAD+ in Drosophila melanogaster. Mech Ageing Dev 56: 223–235
Sohal RS, Mockett RJ and Orr WC (2002) Mechanisms of aging: an appraisal of the oxidative stress hypothesis. Free Rad Biol Med 33: 575–586
Sun J and Tower J (1999) FLP recombinase-mediated induction of Cu/Zn-superoxide dismutase transgene expression can extend the life span of adult Drosophila melanogaster flies. Mol Cell Biol 19: 216–228
Sun J, Folk D, Bradley TJ and Tower J (2002) Induced overexpression of mitochondrial Mn-superoxide dismutase extends the life span of adult Drosophila melanogaster. Genetics 161: 661–672
Tatar M, Bartke A and Antebi A (2003) The endocrine regulation of aging by insulin-like signals. Science 299: 1346–1351
Vieira C, Pasyukova EG, Zeng ZB, Hackett JB, Lyman RF and Mackay TEC (2000) Genotype-environment interaction for quantitative trait loci affecting life span in Drosophila melanogaster. Genetics 154: 213–227
Wade CE, Moran MM and Oyama J (2002) Resting energy expenditure of rats acclimated to hypergravity. Aviat Space Environ Med 73: 859–864
Xia E, Rao G, van Remmen H, Heydari AR and Richardson A (1995) Activities of antioxidant enzymes in various tissues of male Fischer 344 rats are altered by food restriction. J Nutr 125: 195–201
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Bourg, É.L., Fournier, D. Is Lifespan Extension Accompanied by Improved Antioxidant Defences? A Study of Superoxide Dismutase and Catalase in Drosophila Melanogaster Flies that Lived in Hypergravity at a Young Age. Biogerontology 5, 261–266 (2004). https://doi.org/10.1023/B:BGEN.0000038046.37590.03
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DOI: https://doi.org/10.1023/B:BGEN.0000038046.37590.03