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Increased Catalase Activity in Mouse Cell Mutants Resistant to Paraquat

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Abstract

Paraquat is widely used as a generator of superoxide radicals in a cell. First it was shown to immediately induce premature senescence in normal human fibroblasts. To assess its defense mechanisms, we characterized three paraquat-resistant mutants from mouse FM3A cells, MPQR40-1 and MPQR40-3 isolated by a single-step selection after mutagenesis, and SPQR100-6 isolated spontaneously. All exhibited six to eight times more resistance to paraquat than the parental line. In cell—cell hybrids, their phenotypes were reccessive, co-dominant, or dominant, respectively. Biochemical characterization revealed that activity and mRNA level for catalase were increased in all of the mutants. In MPQR40-3 that showed the highest degree of paraquat resistance, total SOD activity and mRNA level for Cu/Zn—SOD were also increased. These results suggest that catalase plays a major role in paraquat resistance in mouse FM3A cells.

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Authors and Affiliations

  1. Graduate School of Integrated Science and Kihara Institute for Biological Research, Yokohama City University, Maioka-cho 641-12, Totsuka-ku, Yokohama, 244-0813, Japan

    Atsuhiro Joguchi, Michihiko Fujii & Dai Ayusawa

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  1. Atsuhiro Joguchi
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  2. Michihiko Fujii
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  3. Dai Ayusawa
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Correspondence to Dai Ayusawa.

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Joguchi, A., Fujii, M. & Ayusawa, D. Increased Catalase Activity in Mouse Cell Mutants Resistant to Paraquat. Biogerontology 5, 193–200 (2004). https://doi.org/10.1023/B:BGEN.0000031157.12946.e3

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  • DOI: https://doi.org/10.1023/B:BGEN.0000031157.12946.e3

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