Abstract
The effects of 5-fold injections of normal saline were studied in Wistar rats in two conditioned reflex situations: defense and alimentary. Intact animals developed conditioned pain avoidance reflex in repeated tail-flick test after 60 presentations over 3 days. Two injections of normal saline were sufficient for the formation of a stable negative stress status in experimental rats, which manifested by a drastic increase in tactile sensitivity persisting for 5 days after discontinuation of injections. Only 15% experimental rats vs. 40% intact animals developed the food-procuring response in a multialternative maze. Presumably, repeated injections of normal saline should be regarded as a potent negative stress factor modulating animals behavior, and these signs should be taken into consideration, when interpreting the effects observed in pharmacological investigations.
Similar content being viewed by others
REFERENCES
O. V. Eshchenko, K. A. Nikol'skaya, V. I. Deigin, and E. P. Yarova, Byull. Eksp. Biol. Med., 126, No. 8, 425–427 (1998).
N. A. Krupina, I. N. Orlova, and G. N. Kryzhanovskii, Ibid., 120, No. 7, 66–71 (1995).
N. A. Krupina, I. N. Orlova, and G. N. Kryzhanovskii, Ibid., No. 8, 160–164.
K. A. Nikol'skaya, A. V. Savonenko, A. I. Osipov, et al., Uspekhi Sovrem. Biol., 115, No. 4, 390–396 (1995).
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kondashevskaya, M.V., Nikolskaya, K.A. Active Control and Its Stress Effects. Bulletin of Experimental Biology and Medicine 138, 207–209 (2004). https://doi.org/10.1023/B:BEBM.0000048391.44182.a7
Issue Date:
DOI: https://doi.org/10.1023/B:BEBM.0000048391.44182.a7