Abstract
The actual size and sites for puncture of the cisterna magna in adult Wistar rats were estimated on acrylic models. We proposed a new method for obtaining cerebrospinal fluid from narcotized rats. This method consists in controlled introduction of a needle into the cisterna magna through the skin and/or dura mater above this structure using a stereotactic micromanipulator. Experiments on rats with focal and global cerebral ischemia showed that this technique ensures single and repeated sampling of at least 50 μl liquor not containing blood admixtures.
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Lebedev, S.V., Blinov, D.V. & Petrov, S.V. Spatial Characteristics of Cisterna Magna in Rats and Novel Technique for Puncture with a Stereotactic Manipulator. Bulletin of Experimental Biology and Medicine 137, 635–638 (2004). https://doi.org/10.1023/B:BEBM.0000042732.00810.01
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DOI: https://doi.org/10.1023/B:BEBM.0000042732.00810.01