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Apoptotic volume decrease, pH acidification and chloride channel activation during apoptosis requires CD45 expression in HPB-ALL T cells

Apoptosis volume 9, pages 543–551 (2004)Cite this article

Abstract

Mitochondrial-perturbating agents such as toxic coumponds induce apoptosis. We note that the loss of CD45 expression in the lymphoblastic leukemia cell line HPB-ALL (HPB45.0) leads to an inhibition of nuclear apoptosis. Our hypothesis is that the absence of CD45 disturbs protein function regulated by a proto-oncogene of the Src family playing a significant role in nuclear apoptosis. In this work we explore the importance of a chloride efflux on DNA fragmentation. The role of tyrosine kinase in the function and regulation of the chloride channels was determined. Our results showed a disturbance of ionic homeostasis in CD45 deficient lymphocytes (CD45−) in contrast to normal lymphocytes (CD45+). The phosphorylation levels of the chloride channels are considerably inhibited in CD45−, while the expression levels of these channels are similar in the two types of cells. A hypertonic medium inhibits DNA fragmentation in CD45+ while a hypotonic medium increases DNA fragmentation in CD45−. Thus CD45 plays a significant role in nuclear apoptosis by the regulation of the chloride channels responsible for ionic homeostasis of the cell essential for the DFF40 activation.

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Affiliations

  1. INRS-Institut Armand-Frappier, Pointe-Claire, Quebec, Canada, H9R 1G6

    G. Dupéré-Minier, C. Hamelin, P. Desharnais & J. Bernier

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  1. G. Dupéré-Minier
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  2. C. Hamelin
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  3. P. Desharnais
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  4. J. Bernier
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Correspondence to J. Bernier.

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Dupéré-Minier, G., Hamelin, C., Desharnais, P. et al. Apoptotic volume decrease, pH acidification and chloride channel activation during apoptosis requires CD45 expression in HPB-ALL T cells. Apoptosis 9, 543–551 (2004). https://doi.org/10.1023/B:APPT.0000038031.84705.84

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  • DOI: https://doi.org/10.1023/B:APPT.0000038031.84705.84

  • apoptosis
  • CD45
  • chloride channel
  • DNA fragmentation
  • ionic regulation
  • Src family kinases