Abstract
To elucidate the mechanisms of localized genesis and development of atherosclerosis and anastomotic intimal hyperplasia in man, a coculture of bovine aortic endothelial cells (ECs) and smooth muscle cells (SMCs) was prepared, and the effects of a shear flow on the uptake of lipoproteins by the cells was studied by incubating the EC–SMC coculture as well as an EC monoculture with a culture medium containing either DiI-LDL or DiI-Ac-LDL and subjecting to a laminar shear flow. It was found that in both the presence and absence of a shear flow that imposed the ECs an area mean shear stress of 13.3 dynes/cm2, the uptake of LDL by an EC–SMC coculture was much greater than that by an EC monoculture, whereas that of Ac-LDL was almost the same. The uptake of LDL by an EC monoculture increased slightly by being exposed to a shear flow, whereas that by an EC–SMC coculture did not. In contrast to this, the uptake of Ac-LDL by both an EC monoculture and an EC–SMC coculture decreased drastically by a shear flow, suggesting that the action of a shear flow on the uptake of Ac-LDL by vascular cells is very different from that of LDL.
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Niwa, K., Kado, T., Sakai, J. et al. The Effects of a Shear Flow on the Uptake of LDL and Acetylated LDL by an EC Monoculture and an EC–SMC Coculture. Annals of Biomedical Engineering 32, 537–543 (2004). https://doi.org/10.1023/B:ABME.0000019173.79939.54
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DOI: https://doi.org/10.1023/B:ABME.0000019173.79939.54