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Production of Surfactants by Rhodococcus erythropolis Strain EK-1, Grown on Hydrophilic and Hydrophobic Substrates

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Abstract

The ability of Rhodococcus erythropolis strain EK-1 to produce surfactants when grown on hydrophilic (ethanol and glucose) and hydrophobic (liquid paraffins and hexadecane) substrates was studied. The strain was found to produce surfactants with emulsifying and surface-active properties. The production of surfactants depended on the composition of the nutritive medium, nature and concentration of the sources of carbon and nitrogen, and duration of cultivation. Chemically, surfactants produced by Rhodococcus erythropolis EK-1 grown on ethanol are a complex of lipids with polysaccharide–proteinaceous substances. The lipids include glycolipids (trehalose mono- and dicorynomycolates) and common lipids (cetyl alcohol, palmitic acid, methyl n-pentadecanoate, triglycerides, and mycolic acids).

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REFERENCES

  1. Pirog, T.P., Shevchuk, T.A., Voloshina, I.N., and Gregir-chak, N.N., Prikl. Biokhim. Mikrobiol., 2004 (in press).

  2. Lang, S. and Philp, J.C., Antonie van Leeuwenhoek, 1998, vol. 74, pp. 59–70.

    Google Scholar 

  3. Rosenberg, E. and Ron, E.Z., Appl. Microbiol. Biotech-nol., 1999, vol. 52, pp. 154–162.

    Google Scholar 

  4. Desai, J.D. and Banat, I.M., Microbiol. Mol. Biol. Rev., 1997, vol. 61, no. 1, pp. 47–64.

    PubMed  Google Scholar 

  5. Bouchez-Naitali, M., Rakatozafy, H., Marchal, R., Leveau J.Y., and Vandecasteele J.P, J. Appl. Microbiol., 1999, vol. 86, no. 3, pp. 421–428.

    PubMed  Google Scholar 

  6. Ron, E.Z. and Rosenberg, E., Curr. Opin. Biotechnol., 2002, vol. 13, no. 3, pp. 249–252.

    PubMed  Google Scholar 

  7. Abramzon, A.A., Zaichenko, L.P., and Raingol'd, S.I., Poverkhnostno-aktivnye veshchestva. Sintez, analiz, primenenie (Surfactants: Synthesis, Analysis, and Exploitation), Leningrad: Khimiya, 1988.

    Google Scholar 

  8. Guerra-Santos, Z., Kappeli, O., and Fiechter, A., Appl. Environ. Microbiol., 1984, vol. 48, no. 2, pp. 301–305.

    PubMed  Google Scholar 

  9. Cooper, D.C. and Goldenger, B.G., Appl. Environ. Microbiol., 1987, vol. 53, no. 2, pp. 224–229.

    Google Scholar 

  10. Dubois, M., Gilles, K., Hamilton, J., Rebers, P., and Smith, F., Anal. Chem., 1956, vol. 28, no. 3, pp. 350–356.

    Google Scholar 

  11. Addo, S. and Saito, M., Chromatography Lipid Biomed-ical Research and Chemical Diagnostic, Amsterdam: Elsevier, 1987.

    Google Scholar 

  12. Kuyukina, M.S., Ivchina, I.B., Philp, J.C., Christofi, N., Dunbar, S.A., and Ritchkova, M.I., J. Microbiol. Meth-ods, 2001, vol. 46, pp. 149–156.

    Google Scholar 

  13. Shul'ga, A.N., Karpenko, E.V., Eliseev, S.A., Turovskii, A.A., and Koronelli, T.V., Mikrobiologiya, 1990, vol. 59, no. 3, pp. 443–447.

    Google Scholar 

  14. Philp, J.C., Kuyukina, M.S., Ivshina, I.B., Dunbar, S.A., Christofi, N., Lang, S., and Wray, V., Appl. Microbiol. Biotechnol., 2002, vol. 59, nos. 2/3, pp. 318–324.

    PubMed  Google Scholar 

  15. Mata-Sandoval, J.C., Karns, J., and Torrents, A., Micro-biol. Res., 2001, vol. 155, no. 4, pp. 249–256.

    Google Scholar 

  16. Turkovskaya, O.V., Dmitrieva, T.V., and Muratova, A.Yu., Prikl. Biokhim. Mikrobiol., 2001, vol. 37, no. 1, pp. 80–85.

    PubMed  Google Scholar 

  17. Santa Anna, L.M., Sebastian, G.V., Pereria, N.Jr., Alves, T.L., Menezes, E.P., and Freire, D.M., Appl. Bio-chem. Biotechnol., 2001, vol. 91–93, pp. 459–467.

    Google Scholar 

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Pirog, T.P., Shevchuk, T.A., Voloshina, I.N. et al. Production of Surfactants by Rhodococcus erythropolis Strain EK-1, Grown on Hydrophilic and Hydrophobic Substrates. Applied Biochemistry and Microbiology 40, 470–475 (2004). https://doi.org/10.1023/B:ABIM.0000040670.33787.5f

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  • DOI: https://doi.org/10.1023/B:ABIM.0000040670.33787.5f

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